Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab, India.
J Biophotonics. 2024 Mar;17(3):e202300423. doi: 10.1002/jbio.202300423. Epub 2023 Dec 22.
Flow cytometry is a potent method that enables the quick and concurrent investigation of several characteristics of single cells in solution. Photodiodes or photomultiplier tubes are employed to detect the dispersed and fluorescent light signals that are produced by the laser beam as it passes through the cells. Photodetectors transform the light signals produced by the laser into electrical impulses. A computer then analyses these electrical impulses to identify and measure the various cell populations depending on their fluorescence or light scattering characteristics. Based on their fluorescence or light scattering properties, cell populations can be examined and/or isolated. This review covers the basic principle, components, working and specific biological applications of flow cytometry, including studies on plant, cell and molecular biology and methods employed for data processing and interpretation as well as the potential future relevance of this methodology in light of retrospective analysis and recent advancements in flow cytometry.
流式细胞术是一种强大的方法，能够快速、同时研究溶液中单细胞的多个特性。光电二极管或光电倍增管用于检测激光束穿过细胞时产生的离散和荧光光信号。光电探测器将激光产生的光信号转换成电脉冲。然后，计算机分析这些电脉冲，根据细胞的荧光或光散射特性来识别和测量各种细胞群体。根据其荧光或光散射特性，可以对细胞群体进行检查和/或分离。本文综述了流式细胞术的基本原理、组成、工作原理和特定的生物学应用，包括植物、细胞和分子生物学的研究，以及数据处理和解释所采用的方法，以及鉴于回顾性分析和流式细胞术的最新进展，该方法未来的潜在相关性。
