Vago P, Bureau J P
Laboratoire de biologie cellulaire et d'immunogénétique, Faculté de Médecine, Nimes.
Ann Pathol. 1989;9(4):289-94.
Through flow cytometry cells are investigated by optical, electrical and electronic components. On a rate of several hundred cells per second, quantitative multiparametric cells studies are realized on a cell by cell basis. Cells in monodisperse suspension are drawn by a stream of fluid which aligns them before their interception by a beam of light, generally produced by Laser. The generated optical signals (diffused lights and emitted fluorescences), related to biological properties of the cells, are conveyed to photomultipliers which transform light energy into electric currents. The signals are quantified by electronic and processing components which can drive cell sorting. Flow cytometry is playing an increasingly significant role in the practice of the clinical laboratory in several areas i.e. oncology (DNA content measurement) or immunology (lymphocytes phenotyping).
通过流式细胞仪，利用光学、电学和电子元件对细胞进行研究。每秒可检测数百个细胞，在逐个细胞的基础上实现定量多参数细胞研究。单分散悬浮液中的细胞被一股流体吸引，在被通常由激光产生的光束拦截之前使它们排列整齐。与细胞生物学特性相关的产生的光信号（散射光和发射荧光）被传送到光电倍增管，光电倍增管将光能转化为电流。信号由可驱动细胞分选的电子和处理元件进行量化。流式细胞术在临床实验室实践的几个领域，即肿瘤学（DNA含量测量）或免疫学（淋巴细胞表型分析）中发挥着越来越重要的作用。
