Higley N A, Beery J T, Taylor S L, Porter J W, Dziuba J A, Lalich J J
Atherosclerosis. 1986 Nov;62(2):91-104. doi: 10.1016/0021-9150(86)90053-5.
Previous findings indicating that the oxidation products of cholesterol are associated with atherogenicity have led to a comparative study of the subchronic effects of feeding rabbits purified cholesterol, oxidized cholesterols free of cholesterol and cholesterol esters, or a mixture of cholesterol and oxidized cholesterols. Macroscopically, the cholesterol-fed animals exhibited 6-fold more arterial lesions than the animals fed cholesterol-free oxidized cholesterols. Microscopically, there was no statistically significant difference from the control in the number of histochemically-defined lesions in any of the groups. However, the lesions in the cholesterol-fed group were more severe, as indicated by a statistically significant increase in the magnitude of the lesions. This increased severity was also characterized by greater frequency and intensity of Azure A/Thionin, VonKossa, and Horseradish Peroxidase-Wheat Germ Agglutinin staining. Electron-microscopic studies of normal appearing arterial tissues showed an increased density of viable smooth muscle cells and an increase in vacuolar extracellular debris in the cholesterol-fed group. Oxidized cholesterols in the concentrations and relative compositions administered here are markedly less atherogenic to rabbits than highly purified cholesterol.
先前的研究结果表明，胆固醇的氧化产物与动脉粥样硬化性有关，这促使人们对给兔子喂食纯化胆固醇、不含胆固醇和胆固醇酯的氧化胆固醇或胆固醇与氧化胆固醇的混合物的亚慢性影响进行了比较研究。从宏观上看，喂食胆固醇的动物出现的动脉病变比喂食不含胆固醇的氧化胆固醇的动物多5倍。在显微镜下，任何一组中通过组织化学定义的病变数量与对照组相比均无统计学上的显著差异。然而，喂食胆固醇组的病变更为严重，这表现为病变程度在统计学上显著增加。这种增加的严重程度还表现为天青A/硫堇、冯科萨染色以及辣根过氧化物酶-小麦胚芽凝集素染色的频率和强度更高。对外观正常的动脉组织进行的电子显微镜研究显示，喂食胆固醇组中存活的平滑肌细胞密度增加，且空泡状细胞外碎片增多。在此所给予的浓度和相对组成下的氧化胆固醇对兔子的致动脉粥样硬化性明显低于高度纯化的胆固醇。
