Tipton C L, Leung P C, Johnson J S, Brooks R J, Beitz D C
Biochem Biophys Res Commun. 1987 Aug 14;146(3):1166-72. doi: 10.1016/0006-291x(87)90770-4.
A mixture of cholesterol autoxidation products, prepared from an aged sample of cholesterol by recrystallization from methanol, inhibits calmodulin irreversibly in a Ca2+-dependent reaction. Inhibitory activity is lost after treatment with NaBH4, NaCNBH3, or NaI, from which we conclude that calmodulin inhibition is due to one or more cholesterol hydroperoxides. Partially purified cholesterol hydroperoxides, with or without cholesterol, were fed to young adult white rabbits. Cholesterol in the diet caused extensive atheroma formation in the aortas, but the addition of cholesterol hydroperoxides markedly reduced lesion formation. A cholesterol hydroperoxide preparation that was reduced by treatment with NaI was not effective in preventing atheroma formation. Cholesterol hydroperoxides did not lower cholesterol concentrations in blood plasma, liver, or heart.
由胆固醇老化样品经甲醇重结晶制备的胆固醇自氧化产物混合物，在Ca2+依赖性反应中不可逆地抑制钙调蛋白。用NaBH4、NaCNBH3或NaI处理后抑制活性丧失，由此我们得出结论，钙调蛋白抑制是由于一种或多种胆固醇氢过氧化物。将部分纯化的胆固醇氢过氧化物（含或不含胆固醇）喂给成年白兔。饮食中的胆固醇导致主动脉中广泛形成动脉粥样瘤，但添加胆固醇氢过氧化物可显著减少病变形成。经NaI处理而还原的胆固醇氢过氧化物制剂在预防动脉粥样瘤形成方面无效。胆固醇氢过氧化物不会降低血浆、肝脏或心脏中的胆固醇浓度。
