Jiang Wenbin, Lu Changhao, Fan Jinjuan, Wu Mengyang, Chen Wenlong, Zhao Degang, Niu Suzhen, He Yingqin
College of Tea Science, Guizhou University, Guiyang 550025, China.
Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Scientific Observing and Experimental Station of Crop Pest in Guiyang, Ministry of Agriculture, Guizhou University, Guiyang 550025, China.
J Econ Entomol. 2024 Feb 12;117(1):302-310. doi: 10.1093/jee/toad220.
Toxoptera aurantii is one of the most destructive pests, threatening the yield and quality of tea plantations. The salicylic acid (SA)-mediated signaling pathway is vital for the induction of plant defense responses; however, its role in tea plant resistance to T. aurantii remains unclear. Thus, this study used and electrical penetration graph and monitoring of population dynamics to evaluate the effects of exogenous SA application on T. aurantii feeding behavior and population growth in tea seedlings. Moreover, the effects of SA treatment on the activities of defense-related enzymes were analyzed. Probe counts and the duration of xylem sap ingestion were significantly higher in SA-treated plants than those in the control group. The total duration of passive phloem ingestion was significantly decreased in 0.5 mmol/l SA-treated plants, and the application of 0.5, 1, and 4 mmol/l SA significantly inhibited T. aurantii population growth. In addition, the activities of polyphenol oxidase, peroxidase, and superoxide dismutase were significantly increased in the 0.5 mmol/l SA-treated plants. Overall, this study demonstrates the capacity of exogenous SA to activate defense responses against T. aurantii. These results have crucial implications for understanding the mechanisms of enhanced resistance, thereby providing a sustainable approach for managing T. aurantii.
橘蚜是最具破坏性的害虫之一，威胁着茶园的产量和品质。水杨酸（SA）介导的信号通路对于诱导植物防御反应至关重要；然而，其在茶树对橘蚜抗性中的作用仍不清楚。因此，本研究采用刺探电位图谱和种群动态监测来评估外源SA处理对橘蚜取食行为和茶苗种群增长的影响。此外，还分析了SA处理对防御相关酶活性的影响。SA处理植株的刺探次数和木质部汁液吸食持续时间显著高于对照组。0.5 mmol/l SA处理植株被动韧皮部吸食的总持续时间显著减少，0.5、1和4 mmol/l SA的施用显著抑制了橘蚜种群增长。此外，0.5 mmol/l SA处理植株中多酚氧化酶、过氧化物酶和超氧化物歧化酶的活性显著增加。总体而言，本研究证明了外源SA激活对橘蚜防御反应的能力。这些结果对于理解增强抗性的机制具有关键意义，从而为管理橘蚜提供了一种可持续的方法。
