Department of Clinical Laboratory, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Sci Rep. 2023 Nov 27;13(1):20814. doi: 10.1038/s41598-023-47915-x.
Research has shown that the concentration and composition of biological samples may change after long-term ultra-low temperature storage. Consequently, this study examined the effect of ultra-low temperature storage on serum sIgE detection by comparing sIgE concentrations at various durations from the time of sample storage to subsequent testing. We selected 40 serum samples from the Guangzhou Medical University Affiliated First Hospital Biobank, which had been tested for house dust mites, dog hair, tobacco mold, cockroaches, and cow milk allergen sIgE. Samples were categorized by storage duration: 14 samples stored for 10 years, 12 for 5 years, and 14 for 3 years. They were also classified by sIgE positive levels: 15 samples at levels 1-2, 15 at levels 3-4, and 10 at levels 5-6. The allergen sIgE of these samples was retested using the same technology. Regardless of the type of allergen or the level of positivity, the majority of sIgE concentrations measured at the time of storage were higher than the current measurements, but the difference was not statistically significant. The correlation between the sIgE results at the time of storage and the current results was high for samples stored for 10 years (rs = 0.991, P < 0.001) and 5 years (rs = 0.964, P < 0.001). Serum allergen sIgE is stable when stored under ultra-low temperature conditions, making the construction of a biological sample bank for allergic diseases feasible. This will facilitate researchers in quickly obtaining samples, conducting technical research, and translating findings, thereby promoting the development of the field of allergy through integration of industry, academia, and research.
研究表明，生物样本经过长期超低温储存后，其浓度和组成可能会发生变化。因此，本研究通过比较样本储存至后续检测不同时间点的 sIgE 浓度，来考察超低温储存对血清 sIgE 检测的影响。我们选取了广州医科大学附属第一医院生物样本库中已检测屋尘螨、狗毛、烟曲霉、蟑螂、牛奶过敏原 sIgE 的 40 例血清样本。根据储存时间将样本分为 10 年储存 14 例、5 年储存 12 例、3 年储存 14 例；根据 sIgE 阳性水平将样本分为 1-2 级水平 15 例、3-4 级水平 15 例、5-6 级水平 10 例。采用相同技术对这些样本的过敏原 sIgE 进行复测。无论过敏原类型或阳性水平如何，储存时测量的大多数 sIgE 浓度均高于当前测量值，但差异无统计学意义。储存 10 年（rs=0.991，P<0.001）和 5 年（rs=0.964，P<0.001）样本的 sIgE 结果与当前结果之间的相关性较高。在超低温条件下储存血清过敏原 sIgE 稳定，因此构建过敏性疾病的生物样本库是可行的。这将有助于研究人员快速获取样本，进行技术研究和转化研究，通过产学研一体化推动过敏领域的发展。
