Young Caryl A, Burt Emily, Munnamalai Vidhya
bioRxiv. 2023 Dec 19:2023.11.13.566920. doi: 10.1101/2023.11.13.566920.
During embryonic development Wnt signaling has been shown to influence proliferation and sensory formation in the cochlea. How the dual nature of Wnt signaling is coordinated is unknown. In this study, we define a novel role for a Wnt regulated gene, which was already known to be important for proliferation, in influencing patterning and determining the size of the sensory epithelium in the murine cochlea. Using a quantitative spatial analysis approach and analyzing loss-of-function cochleas, we show that simultaneously specifies the progenitor niche and the size of the sensory domain, and influences the positioning of the medial sensory domain boundary via regulation during the mid-gestational stages. conditional knockout resulted in a decrease of proliferation within the progenitor niche. During the late embryonic stages, conditional knockout of produced a wider sensory epithelium across the radial axis with an increase in ectopic inner hair cell formation. These data suggest that -positive progenitors play a role in boundary formation and patterning the sensory epithelium.
is a Wnt-regulated gene encoding a transcription factor that is expressed in the cochlear progenitor niche and influences the boundary formation between the niche and the sensory domain during mid-cochlear developmental stages, thereby impacting the size of the sensory epithelium.
在胚胎发育过程中，Wnt信号已被证明会影响耳蜗的增殖和感觉形成。Wnt信号的双重性质是如何协调的尚不清楚。在本研究中，我们确定了一个Wnt调控基因的新作用，该基因已知对增殖很重要，它在影响小鼠耳蜗感觉上皮的模式形成和确定其大小方面发挥作用。使用定量空间分析方法并分析功能缺失的耳蜗，我们发现 同时确定了祖细胞龛和感觉域的大小，并在妊娠中期通过 调控影响内侧感觉域边界的定位。 条件性敲除导致祖细胞龛内的增殖减少。在胚胎后期， 的条件性敲除导致整个径向轴上的感觉上皮变宽，异位内毛细胞形成增加。这些数据表明， 阳性祖细胞在感觉上皮的边界形成和模式形成中发挥作用。
是一个Wnt调控基因，编码一种转录因子，该转录因子在耳蜗祖细胞龛中表达，并在耳蜗发育中期影响龛与感觉域之间的边界形成，从而影响感觉上皮的大小。
