Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology, Ministry of Education), Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
China Meat Food Research Center, Beijing Academy of Food Sciences, Beijing 100068, PR China.
Biomater Sci. 2024 Jan 30;12(3):581-595. doi: 10.1039/d3bm01394d.
Recently, nucleic acid delivery has become an amazing route for the treatment of various malignant diseases, and polycationic vectors are attracting more and more attention among gene vectors. However, conventional polycationic vectors still face many obstacles in nucleic acid delivery, such as significant cytotoxicity, high protein absorption behavior, and unsatisfactory blood compatibility caused by a high positive charge density. To solve these problems, the fabrication of hydroxyl-rich branched polycationic vectors has been proposed. For the synthesis of hydroxyl-rich branched polycations, a one-pot method is considered as the preferred method due to its simple preparation process. In this review, typical one-pot methods for fabricating hydroxyl-rich polycations are presented. In particular, amine-epoxide ring-opening polymerization as a novel approach is mainly introduced. In addition, various therapeutic scenarios of hydroxyl-rich branched polycations one-pot fabrication are also generalized. We believe that this review will motivate the optimized design of hydroxyl-rich branched polycations for potential nucleic acid delivery and their bio-applications.
最近，核酸递送已成为治疗各种恶性疾病的一种令人惊叹的途径，其中聚阳离子载体在基因载体中越来越受到关注。然而，传统的聚阳离子载体在核酸递送上仍然面临许多障碍，如由于正电荷密度高而导致的显著细胞毒性、高蛋白吸收行为和不理想的血液相容性。为了解决这些问题，人们提出了制备富含羟基的支化聚阳离子载体。对于富含羟基的支化聚阳离子的合成，由于其制备工艺简单，一锅法被认为是首选方法。在这篇综述中，介绍了典型的一锅法制备富含羟基的聚阳离子的方法。特别是，主要介绍了胺-环氧化物开环聚合这一新颖方法。此外，还概括了富含羟基的支化聚阳离子一锅法制备的各种治疗应用场景。我们相信，这篇综述将激励对富含羟基的支化聚阳离子进行优化设计，以用于潜在的核酸递送及其生物应用。
