Chefurka W, Chatelier R C, Sawyer W H
Biochim Biophys Acta. 1987 Jan 26;896(2):181-6. doi: 10.1016/0005-2736(87)90178-7.
The localization of the effects of DDT (5-50 mol%) addition on the acyl chain dynamics in unilamellar vesicles of two phosphatidylcholines (DPPC and egg PC) has been investigated by steady-state fluorescence polarization of a series of n-(9-anthroyloxy) fatty acids (n = 2, 6, 9, 12 and 16) whose fluorophore is located at a graded series of depths from the surface to the centre of the bilayer. The results show that DDT is a fluidizer of DPPC and egg PC bilayers. The increase in microviscosity of DPPC bilayers at 23 degrees C begins at the centre of the bilayer (5 mol% DDT) and proceeds outward to the surface with increasing concentration of DDT (17 mol%). This pattern of effects is not evident in fluid bilayers of DPPC at 54 degrees C or egg PC at 23 degrees C. DDT (33 mol%) also lowers the phase transition temperature of DPPC bilayers by approximately 2 Cdeg. DDT (17 mol%) had no effect on the mean excited fluorescence life-time of 2-AP and 12-AS in DPPC, DOPC and egg PC bilayers. No quenching of 2-AP fluorescence was evident.
通过一系列n -（9 - 蒽氧基）脂肪酸（n = 2、6、9、12和16）的稳态荧光偏振研究了添加滴滴涕（5 - 50摩尔%）对两种磷脂酰胆碱（二棕榈酰磷脂酰胆碱和蛋黄卵磷脂）单层囊泡中酰基链动力学的影响定位，这些脂肪酸的荧光团位于从双层表面到中心的一系列分级深度处。结果表明，滴滴涕是二棕榈酰磷脂酰胆碱和蛋黄卵磷脂双层的流化剂。在23℃时，二棕榈酰磷脂酰胆碱双层微粘度的增加始于双层中心（5摩尔%滴滴涕），并随着滴滴涕浓度增加（17摩尔%）向外延伸至表面。在54℃的二棕榈酰磷脂酰胆碱流体双层或23℃的蛋黄卵磷脂流体双层中，这种效应模式不明显。滴滴涕（33摩尔%）还使二棕榈酰磷脂酰胆碱双层的相变温度降低约2℃。滴滴涕（17摩尔%）对二棕榈酰磷脂酰胆碱、二油酰磷脂酰胆碱和蛋黄卵磷脂双层中2 - 氨基芘和12 - 硬脂酸的平均激发荧光寿命没有影响。未观察到2 - 氨基芘荧光的猝灭。
