Stone G R, Baldwin J P, Carpenter B G
Biochim Biophys Acta. 1987 Jan 28;908(1):34-45. doi: 10.1016/0167-4781(87)90019-4.
The inherent instability of Physarum nucleosome core particles prepared by micrococcal nuclease digestion in Na+/Ca2+ buffers can be overcome by the addition of 0.15 mM spermine and 0.5 mM spermidine. Neutron scattering, circular dichroism, nuclease digestion and thermal denaturation studies carried out on these stable monosomes show them to be very similar to those obtained from higher eukaryotes.
通过在Na⁺/Ca²⁺缓冲液中用微球菌核酸酶消化制备的黏菌核小体核心颗粒的固有不稳定性，可以通过添加0.15 mM精胺和0.5 mM亚精胺来克服。对这些稳定的单核小体进行的中子散射、圆二色性、核酸酶消化和热变性研究表明，它们与从高等真核生物中获得的核小体非常相似。
