Johnson E M, Littau V C, Allfrey V G, Bradbury E M, Matthews H R
Nucleic Acids Res. 1976 Dec;3(12):3313-29. doi: 10.1093/nar/3.12.3313.
Nucleosome DNA repeat lengths in Physarum chromatin, determined by nuclease digestion experiments, are shorter than those observed in most mammalian chromatin and longer than those reported for chromatin of certain other lower eukaryotes. After digestion with staphylococcal nuclease for short periods of time an average repeat length of 190 base pairs is measured. After more extensive digestion an average repeat length of 172 base pairs is measured. Upon prolonged digestion DNA is degraded to an average monomer subunit length of 160 base pairs, with only a small amount of DNA found in lengths of 130 base pairs or smaller. Mathematical analysis of the data suggests that the Physarum nucleosome DNA repeat comprises a protected DNA segment of about 159 base pairs with a nuclease-accessible interconnecting segment which ranges from 13 to 31 base pairs. The spacing data are compatible with measurements from electron micrographs of Physarum chromatin.
通过核酸酶消化实验测定，绒泡菌染色质中的核小体DNA重复长度比大多数哺乳动物染色质中的短，但比某些其他低等真核生物染色质中报道的长。用葡萄球菌核酸酶短时间消化后，测得平均重复长度为190个碱基对。经过更广泛的消化后，测得平均重复长度为172个碱基对。长时间消化后，DNA降解为平均单体亚基长度为160个碱基对，只有少量DNA长度为130个碱基对或更短。对数据的数学分析表明，绒泡菌核小体DNA重复序列包含一个约159个碱基对的受保护DNA片段，以及一个核酸酶可及的13至31个碱基对的连接片段。间距数据与绒泡菌染色质电子显微镜测量结果相符。
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