School of Chemistry, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Australia Centre for Nanomedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Analyst. 2024 Jan 15;149(2):269-289. doi: 10.1039/d3an01423a.
Herein the development of cellular impedance biosensors, electrochemical impedance spectroscopy, and the general principles and terms associated with the cell-electrode interface is reviewed. This family of techniques provides quantitative and sensitive information into cell responses to stimuli in real-time with high temporal resolution. The applications of cell-based impedance biosensors as a readout in cell biology is illustrated with a diverse range of examples. The current state of the field, its limitations, the possible available solutions, and the potential benefits of developing biosensors are discussed.
本文综述了细胞阻抗生物传感器的发展、电化学阻抗谱以及与细胞-电极界面相关的一般原理和术语。该技术家族可实时提供定量且灵敏的细胞响应刺激信息，具有高时间分辨率。通过各种示例说明了基于细胞的阻抗生物传感器作为细胞生物学读出的应用。讨论了该领域的现状、其局限性、可能的解决方案以及开发生物传感器的潜在好处。
