Kabishev A A, Nechaev V N, Riazanova L A, Ptitsin L R, Batchikova N V
Biokhimiia. 1986 Nov;51(11):1821-8.
Using two consecutive oligo(dT)-cellulose column chromatography steps, the total poly(A)RNA was isolated from the livers of rats injected with phenobarbital (PB) or 3-methylcholanthrene (MC). During translation of the PB-induced mRNA in the reticulocyte lysate cell-free protein-synthesizing system, a single polypeptide with an apparent molecular weight of 50,000 was synthesized which was specifically immunoprecipitated by antibodies to major PB-inducible cytochrome P-450 PB-3. In contrast, after completion of MC-mRNA translation, the antibodies to major MC-induced cytochrome MC-2 precipitated from the incubation mixture 4-5 polypeptides, of which the largest one with an apparent molecular weight of 58,000 corresponded to cytochrome P-450 MC-2. During sucrose density gradient centrifugation, the PB- and MS-mRNAs with sedimentation coefficients of about 18S and 20S, respectively, were precipitated.
通过连续两步寡聚（dT）-纤维素柱层析，从注射苯巴比妥（PB）或3-甲基胆蒽（MC）的大鼠肝脏中分离出总多聚腺苷酸RNA。在无细胞蛋白质合成系统的网织红细胞裂解物中翻译PB诱导的mRNA时，合成了一种表观分子量为50,000的单一多肽，该多肽被主要PB诱导的细胞色素P-450 PB-3的抗体特异性免疫沉淀。相比之下，在MC-mRNA翻译完成后，主要MC诱导的细胞色素MC-2的抗体从孵育混合物中沉淀出4-5种多肽，其中最大的一种表观分子量为58,000，对应于细胞色素P-450 MC-2。在蔗糖密度梯度离心过程中，沉降系数分别约为18S和20S的PB-和MS-mRNA沉淀下来。
