Department of Conservative, Collage of Dentistry, Hawler Medical University, Erbil, Iraq.
Medical Analysis Department, Faculty of Applied Science, Tishk International University, Erbil Iraq
Cell Mol Biol (Noisy-le-grand). 2023 Nov 15;69(11):149-154. doi: 10.14715/cmb/2023.69.11.22.
The direct mixing of Nano-hydroxyapatite with Resin Adhesive often leads to weak molecular interactions, which can compromise the dispersion of Nano-hydroxyapatite within the resin adhesive and cause structural damage to the Nano-hydroxyapatite. Therefore, the present study aimed to identify an approach in which Nano-hydroxyapatite is charged or modified prior to its integration into resin adhesive. In this study, Nano-hydroxyapatite was modified using aminopropyltriethoxysilane (APTS), subsequently, the amine groups present on the surface of Nano-hydroxyapatite were additionally modified using succinic anhydride. This modification process resulted in the production of negatively charged Nano-hydroxyapatite - aminopropyltriethoxysilane (n-HA-APTS-SAH). The charged Nano-hydroxyapatite particles were characterized using FTIR and SEM. Subsequently, the charged Nano-hydroxyapatite particles were incorporated into resin dental adhesive. Comprehensive characterization of the composite material was carried out through SEM, FTIR, EDX mapping, and Micro-Raman Spectroscopy. The results revealed the presence of Nano-hydroxyapatite in the mixture and a homogeneous and well-dispersed state with no observable aggregation of Nano-hydroxyapatite particles within the adhesive. Furthermore, the particles still maintain a spherical shape with their sizes falling within the nanoscale range.
纳米羟基磷灰石与树脂胶粘剂直接混合往往会导致分子相互作用较弱，这可能会影响纳米羟基磷灰石在树脂胶粘剂中的分散，并对纳米羟基磷灰石造成结构损伤。因此，本研究旨在寻找一种方法，在将纳米羟基磷灰石整合到树脂胶粘剂之前对其进行充电或改性。在本研究中，使用氨丙基三乙氧基硅烷 (APTS) 对纳米羟基磷灰石进行改性，随后，纳米羟基磷灰石表面的氨基进一步用琥珀酸酐进行改性。这一改性过程产生了带负电荷的纳米羟基磷灰石-氨丙基三乙氧基硅烷 (n-HA-APTS-SAH)。采用傅里叶变换红外光谱 (FTIR) 和扫描电子显微镜 (SEM) 对带电荷的纳米羟基磷灰石颗粒进行了表征。随后，将带电荷的纳米羟基磷灰石颗粒掺入树脂牙科胶粘剂中。通过扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR)、能谱 (EDX) 映射和微拉曼光谱对复合材料进行了全面表征。结果表明，混合物中存在纳米羟基磷灰石，且呈均匀分散状态，胶粘剂中未观察到纳米羟基磷灰石颗粒的聚集。此外，颗粒仍保持球形，尺寸仍在纳米范围内。
