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氨丙基三乙氧基硅烷介导的羟基磷灰石纳米粒子的表面功能化：合成、表征和体外毒性试验。

Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay.

机构信息

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai, People's Republic of China.

出版信息

Int J Nanomedicine. 2011;6:3449-59. doi: 10.2147/IJN.S27166. Epub 2011 Dec 20.

DOI:10.2147/IJN.S27166
PMID:22267929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3260038/
Abstract

BACKGROUND

We report on aminopropyltriethoxysilane (APTS)-mediated surface modification of nanohydroxyapatite with different surface functional groups for potential biomedical applications. In this study, nanohydroxyapatite covalently linked with APTS (n-HA-APTS) was reacted with acetic anhydride or succinic anhydride to produce neutralized (n-HA-APTS. Ac) or negatively charged (n-HA-APTS.SAH) nanohydroxyapatite, respectively. Nanohydroxyapatite formed with amine, acetyl, and carboxyl groups was extensively characterized using Fourier transform infrared spectroscopy, transmission electron microscopy, (1)H nuclear magnetic resonance spectroscopy, X-ray diffraction, inductively coupled plasma-atomic emission spectroscopy, and zeta potential measurements.

RESULTS

In vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay revealed that the slight toxicity of the amine-functionalized n-HA-APTS could be eliminated by post-functionalization of APTS amines to form acetyl and carboxyl groups. Blood compatibility assessment demonstrated that the negligible hemolytic activity of the pristine nanohydroxyapatite particles did not appreciably change after APTS-mediated surface functionalization.

CONCLUSION

APTS-mediated functionalization of nanohydroxyapatite with different surface groups may be useful for further functionalization of nanohydroxyapatite with biologically active materials, thereby providing possibilities for a broad range of biomedical applications.

摘要

背景

我们报告了氨丙基三乙氧基硅烷 (APTS) 介导的不同表面官能团纳米羟基磷灰石的表面改性，用于潜在的生物医学应用。在这项研究中，通过与乙酸酐或丁二酸酐反应，将共价连接 APTS 的纳米羟基磷灰石（n-HA-APTS）分别转化为中和的（n-HA-APTS.Ac）或带负电荷的（n-HA-APTS.SAH）纳米羟基磷灰石。使用傅里叶变换红外光谱、透射电子显微镜、（1）H 核磁共振光谱、X 射线衍射、电感耦合等离子体原子发射光谱和 ζ 电位测量对具有胺、乙酰基和羧基的纳米羟基磷灰石进行了广泛的表征。

结果

体外 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴化物比色法显示，APTS 胺的后功能化形成乙酰基和羧基可以消除胺功能化的 n-HA-APTS 的轻微毒性。血液相容性评估表明，原始纳米羟基磷灰石颗粒的可忽略的溶血活性在 APTS 介导的表面官能化后没有明显变化。

结论

APTS 介导的不同表面基团纳米羟基磷灰石的功能化可能有助于进一步对纳米羟基磷灰石进行生物活性材料的功能化，从而为广泛的生物医学应用提供可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebad/3260038/5c1052a4fa94/ijn-6-3449f8.jpg
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