Department of Zoology, University of Delhi, Delhi 110007, India.
Department of Zoology, University of Delhi, Delhi 110007, India.
Comp Biochem Physiol A Mol Integr Physiol. 2024 Feb;288:111556. doi: 10.1016/j.cbpa.2023.111556. Epub 2023 Nov 26.
Nesfatin-1 has recently emerged as a modulator of ovarian functions in mammals. Studies in non-mammalian vertebrates, though limited and majorly restricted to fishes, have evidenced a role of this peptide in the regulation of ovarian steroidogenesis and oocyte maturation. Interestingly, nesfatin-1 remains completely unexplored in reptiles. Therefore, the present study aimed to identify nesfatin-1 and elucidate its role and regulation in the ovary of Hemidactylus flaviviridis. Ovarian expression of nucb2/nesfatin-1 was highest during late recrudescence and breeding while it was lowest during regression. Follicular stage-dependent expression analysis showed significantly high expression of nucb2/nesfatin-1 in previtellogenic follicles. Further, in vitro treatment of recrudescent wall lizard ovaries with nesfatin-1 resulted in increased expression of anti-apoptotic gene, bcl-2, along with a concomitant decline in the pro-apoptotic gene, caspase-3. In addition, proliferation/differentiation markers like scf, c-kit, pcna, and bmp-15 were stimulated in ovaries incubated with the peptide. Ovarian steroidogenesis was also positively influenced by nesfatin-1 as treatment with the peptide resulted in heightened star expression as well as increased estradiol and progesterone production. Also, all concentrations of nesfatin-1 stimulated glucose uptake and metabolism in wall lizard ovary. Our observations provide the first evidence of ovarian functions of nesfatin-1 in a reptile. Further, ovarian nucb2/nesfatin-1 was differentially regulated by gonadotropin and sex steroids wherein its expression was stimulated by dihydrotestosterone (DHT) and 17β-estradiol (E) but inhibited by follicle-stimulating hormone (FSH). In summary, this is the first report of the presence, reproductive stage-dependent expression, role, and regulation of ovarian nucb2/nesfatin-1 in H. flaviviridis.
内脂素-1 最近被认为是哺乳动物卵巢功能的调节剂。虽然非哺乳动物脊椎动物的研究有限，主要限于鱼类，但该肽在调节卵巢甾体生成和卵母细胞成熟方面的作用已得到证实。有趣的是，内脂素-1 在爬行动物中仍然完全未知。因此，本研究旨在鉴定内脂素-1 并阐明其在黄缘闭壳龟卵巢中的作用和调节作用。Nucb2/nesfatin-1 在晚期复苏和繁殖期间卵巢表达最高，而在退化期间表达最低。卵泡阶段依赖性表达分析显示，在卵黄前卵泡中 nucb2/nesfatin-1 表达显著升高。此外，用内脂素-1 处理复苏的壁虎卵巢，导致抗凋亡基因 bcl-2 的表达增加，同时促凋亡基因 caspase-3 的表达下降。此外，增殖/分化标志物如 scf、c-kit、pcna 和 bmp-15 在与肽孵育的卵巢中被刺激。内脂素-1 还对卵巢甾体生成产生积极影响，因为用该肽处理导致星表达增加以及雌二醇和孕酮产量增加。此外，内脂素-1 的所有浓度都刺激壁虎卵巢的葡萄糖摄取和代谢。我们的观察结果为内脂素-1 在爬行动物中的卵巢功能提供了第一个证据。此外，卵巢 nucb2/nesfatin-1 被促性腺激素和性激素差异调节，其表达受二氢睾酮（DHT）和 17β-雌二醇（E）刺激，但受卵泡刺激素（FSH）抑制。总之，这是首次报道 H. flaviviridis 中卵巢 nucb2/nesfatin-1 的存在、生殖阶段依赖性表达、作用和调节。
