Dotania Krittika, Tripathy Mamta, Rai Umesh
Department of Zoology, University of Delhi, Delhi, 110007, India.
University of Jammu, Jammu and Kashmir, 180006, India.
Sci Rep. 2024 Dec 30;14(1):31565. doi: 10.1038/s41598-024-74371-y.
Nesfatin-1 is a crucial regulator of energy homeostasis in mammals and fishes, however, its metabolic role remains completely unexplored in amphibians, reptiles, and birds. Therefore, present study elucidates role of nesfatin-1 in glucose homeostasis in wall lizard wherein fasting stimulated hepatic nucb2/nesfatin-1, glycogen phosphorylase (glyp), phosphoenolpyruvate carboxykinase (pepck), and fructose 1,6-bisphosphatase (fbp), while feeding upregulated pancreatic nucb2/nesfatin-1 and insulin, suggesting towards tissue-specific dual role of nesfatin-1 in glucoregulation. The glycogenolytic/gluconeogenic role of nesfatin-1 was further confirmed by an increase in media glucose levels along with heightened hepatic pepck and fbp expression and concomitant decline in liver glycogen content in nesfatin-1-treated liver of wall lizard. Moreover, treatment with nesfatin-1 stimulated insulin expression in pancreas while insulin downregulated pancreatic nucb2/nesfatin-1. Further, prolonged fasting induced elevated nucb2/nesfatin-1, and lipolytic markers, adipose triglyceride lipase (atgl) and monoglyceride lipase (mgl) in adipose tissue implicate nesfatin-1 in lipolysis which is substantiated by nesfatin-1-mediated direct upregulation of atgl and mgl. Our study provides the first comprehensive overview of tissue-dependent role of nesfatin-1 in regulating energy homeostasis in a reptile.
Nesfatin-1是哺乳动物和鱼类能量稳态的关键调节因子，然而，其在两栖动物、爬行动物和鸟类中的代谢作用仍完全未被探索。因此，本研究阐明了nesfatin-1在壁蜥葡萄糖稳态中的作用，其中禁食刺激肝脏中的nucb2/nesfatin-1、糖原磷酸化酶（glyp）、磷酸烯醇丙酮酸羧激酶（pepck）和果糖1,6-二磷酸酶（fbp），而进食则上调胰腺中的nucb2/nesfatin-1和胰岛素，这表明nesfatin-1在葡萄糖调节中具有组织特异性双重作用。nesfatin-1的糖原分解/糖异生作用通过壁蜥nesfatin-1处理的肝脏中培养基葡萄糖水平的升高、肝脏pepck和fbp表达的增强以及肝脏糖原含量的相应下降得到进一步证实。此外，nesfatin-1处理刺激胰腺中胰岛素的表达，而胰岛素则下调胰腺中的nucb2/nesfatin-1。此外，长期禁食诱导脂肪组织中nucb2/nesfatin-1以及脂解标志物脂肪甘油三酯脂肪酶（atgl）和甘油单酯脂肪酶（mgl）升高，这表明nesfatin-1参与脂解作用，nesfatin-1介导的atgl和mgl的直接上调证实了这一点。我们的研究首次全面概述了nesfatin-1在调节爬行动物能量稳态中的组织依赖性作用。
