L-谷氨酰胺酶（EC 3.5.1.2）对多重耐药铜绿假单胞菌感染的抗菌效果。
Anti-microbial efficacy of L-glutaminase (EC 3.5.1.2) against multidrug-resistant Pseudomonas aeruginosa infection.
机构信息
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq.
出版信息
J Antibiot (Tokyo). 2024 Feb;77(2):111-119. doi: 10.1038/s41429-023-00678-z. Epub 2023 Nov 28.
The aims of this study were isolation-purification and characterization of L-glutaminase from L. gasseri BRLHM clinical isolates and investigation of its efficiency as an antimicrobial agent against multidrug-resistant P. aeruginosa. The MICs of L-glutaminase and gentamicin reference were evaluated by the well-diffusion method. The biofilm on the IUD contraceptive was visualized using atomic force microscopy (AFM) image analyses. The purified L-glutaminase possessed significant antimicrobial activity against P. aeruginosa isolates (p < 0.05), and the antibiofilm formation activity of the purified L-glutaminase was stronger than the antibiofilm activity of the referral standard drug, gentamicin (P < 0.05), which were checked by the inhibition of the biofilm formation on the IUD contraceptive device. Investigations indicated that L-glutaminase may have a crucial role in future clinical applications.
本研究的目的是从临床分离株 L. gasseri BRLHM 中分离纯化 L-谷氨酰胺酶，并研究其作为抗多药耐药铜绿假单胞菌的抗菌剂的效率。采用微孔扩散法评估 L-谷氨酰胺酶和庆大霉素参考标准的 MIC。使用原子力显微镜 (AFM) 图像分析可视化宫内节育器避孕的生物膜。纯化的 L-谷氨酰胺酶对铜绿假单胞菌分离株具有显著的抗菌活性（p<0.05），并且纯化的 L-谷氨酰胺酶的抗生物膜形成活性强于参考标准药物庆大霉素（p<0.05），这通过抑制宫内节育器避孕装置上的生物膜形成来检查。研究表明，L-谷氨酰胺酶在未来的临床应用中可能具有重要作用。
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