Optical cell biology group, Instituto Gulbenkian de Ciência, Oerias, Portugal.
MRC Laboratory for Molecular Cell Biology, University College London, London, UK.
Open Biol. 2023 Nov;13(11):230329. doi: 10.1098/rsob.230329. Epub 2023 Nov 29.
This special feature of , titled 'Advances in Quantitative Bioimaging', proposes an overview of the latest advancements in quantitative bioimaging techniques and their wide-ranging applications. The articles cover various topics, including modern imaging methods that enable visualization on a nanoscale, such as super-resolution microscopy and single-particle analysis. These techniques offer unparalleled insights into complex molecular structures and dynamic cellular processes , such as mapping nuclear pore proteins or tracking single histone deposition events throughout the cell cycle. The articles presented in this edition showcase cutting-edge quantitative imaging techniques coupled with advanced computational analysis capable of precisely measuring biological structures and processes. Examples range from correlating calcium release events to underlying protein organization in heart cells to pioneering tools for categorizing changes in microglia morphology under various conditions. This editorial highlights how these advancements are revolutionizing our understanding of living systems, while acknowledging challenges that must be addressed to fully exploit the potential of these emerging technologies, such as improving molecular probes, algorithms and correlation protocols.
本期特刊名为“定量生物成像的进展”，概述了定量生物成像技术的最新进展及其广泛的应用。这些文章涵盖了各种主题，包括能够实现纳米级可视化的现代成像方法，如超分辨率显微镜和单粒子分析。这些技术为复杂的分子结构和动态细胞过程提供了无与伦比的洞察力，例如绘制核孔蛋白图谱或跟踪整个细胞周期中单一组蛋白沉积事件。本期特刊展示了将先进的定量成像技术与能够精确测量生物结构和过程的先进计算分析相结合的最新成果。从将钙释放事件与心脏细胞中潜在的蛋白质组织相关联到开创性的工具，用于在各种条件下对小神经胶质细胞形态的变化进行分类，这些例子不胜枚举。本社论强调了这些进展如何改变我们对生命系统的理解，同时也认识到必须解决一些挑战，以充分利用这些新兴技术的潜力，例如改进分子探针、算法和相关协议。
