Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, Special Administrative Region 999077, China.
J Agric Food Chem. 2023 Dec 13;71(49):19879-19887. doi: 10.1021/acs.jafc.3c06029. Epub 2023 Nov 29.
Seed germination is a vital process in plant development involving dynamic biochemical transformations such as lipid metabolism. However, the spatial distribution and dynamic changes of lipids in different seed compartments during germination are poorly understood. In this study, we employed liquid chromatography/mass spectrometry (LC/MS)-based lipidomics and MALDI mass spectrometry imaging (MSI) to investigate lipid changes occurring in the cotyledon and plumule of mung bean seeds during germination. Lipidomic data revealed that the germination process reduced the levels of many glycerolipids (e.g., triglyceride) and phosphatidylglycerols (e.g., phosphatidylcholine) while increased the levels of lysophospholipids (e.g., lysophosphatidylcholine) in both the cotyledon and plumule. Sphingolipids (e.g., sphingomyelin) displayed altered levels solely in the plumule. Sterol levels increased in the cotyledon but decreased in the plumule. Further imaging results revealed that MALDI-MSI could serve as a supplement and validate LC-MS data. These findings enhance our understanding of the metabolic processes underlying seedling development, with potential implications for crop improvement and seed quality control.
种子萌发是植物发育过程中的一个关键过程，涉及到动态的生化转化，如脂代谢。然而，在种子萌发过程中不同种子隔室中脂质的空间分布和动态变化还知之甚少。在这项研究中，我们采用基于液相色谱/质谱（LC/MS）的脂质组学和 MALDI 质谱成像（MSI）技术研究了绿豆种子子叶和胚芽在萌发过程中脂质的变化。脂质组学数据表明，萌发过程降低了甘油磷脂（如三酰甘油）和磷脂酰甘油（如磷脂酰胆碱）的水平，同时增加了子叶和胚芽中溶血磷脂（如溶血磷脂酰胆碱）的水平。鞘脂（如神经鞘磷脂）仅在胚芽中显示出改变的水平。甾醇水平在子叶中增加，但在胚芽中减少。进一步的成像结果表明，MALDI-MSI 可以作为 LC-MS 数据的补充和验证。这些发现提高了我们对幼苗发育过程中代谢过程的理解，对作物改良和种子质量控制具有潜在的意义。
