Tang Dongyan, Dong Yinmao, Guo Na, Li Li, Ren Hankun
Department of Chemistry, Harbin Institute of Technology, Harbin, 150000, China.
J Sci Food Agric. 2014 Jun;94(8):1639-47. doi: 10.1002/jsfa.6471. Epub 2013 Dec 3.
The mung bean (Vigna radiata) is a key food crop in much of Asia and contains plentiful biological activities to prevent human disease. Mung bean sprouts have more plentiful metabolites and activities after germination.
The metabolite profile of polyphenols in the germination process was described using the methods of ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry and partial least squares discriminant analysis. Sprouts from different periods were clearly discriminated from each other. Eight flavonoids - vitexin, isovitexin, rutin, kaempferol 3-O-rutinoside, isoquercitrin, genistein, daidzein and isorhamnetin - and two phenolic acids - shikimic acid and caffeic acid - were thought to be chemical markers of the sprouts. The method of high-performance liquid chromatography with diode array detection was established to quantitatively analyze the eight chemical markers of flavonoids, and provides good linearity, repeatability, intra- and inter-day precision, accuracy and recovery. The main metabolic and transformation pathways of the polyphenols in the germination process were discussed.
The proposed method is sensitive, rapid and robust. Understanding the complete profile of polyphenol metabolites in the germination process may be useful for better utilizing mung beans sprouts as the raw materials of functional food, health products and cosmetics.
绿豆（Vigna radiata）是亚洲大部分地区的一种关键粮食作物，具有丰富的预防人类疾病的生物活性。绿豆发芽后具有更丰富的代谢产物和活性。
采用超高效液相色谱-飞行时间质谱联用和偏最小二乘判别分析方法描述了发芽过程中多酚的代谢物谱。不同时期的豆芽能够清晰地相互区分。八种黄酮类化合物——牡荆素、异牡荆素、芦丁、山柰酚3-O-芸香糖苷、异槲皮苷、染料木黄酮、大豆苷元和异鼠李素——以及两种酚酸——莽草酸和咖啡酸——被认为是豆芽的化学标志物。建立了二极管阵列检测高效液相色谱法对八种黄酮类化学标志物进行定量分析，具有良好的线性、重复性、日内和日间精密度、准确度和回收率。讨论了发芽过程中多酚的主要代谢和转化途径。
所提出的方法灵敏、快速且稳健。了解发芽过程中多酚代谢物的完整谱图可能有助于更好地将绿豆芽用作功能性食品、保健品和化妆品的原料。
