Department of Medicine, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, East Java, Indonesia.
Department of Pharmacy, Faculty of Medicine and Health Sciences, UIN Maulana Malik Ibrahim Malang, East Java, Indonesia.
Asian Pac J Cancer Prev. 2023 Nov 1;24(11):3783-3794. doi: 10.31557/APJCP.2023.24.11.3783.
The objective of this study was to evaluate the potential and mechanisms of phytochemicals in Eleutherine bulbosa (EBE) in inducing apoptosis and inhibiting the cell cycle in breast cancer through a network pharmacology approach and in vitro validation.
This research employed a literature review approach to identify active anti-cancer compounds and utilized a network pharmacology approach to predict the mechanisms of action of EBE compounds in breast cancer. In addition, in vitro experiments were conducted using MTT method to evaluate the effects of EBE on the cytotoxicity of T47D cells, and the flow cytometry method was employed to determine the impact of EBE on apoptosis and the cell cycle.
The network pharmacology analysis revealed that EBE had an impact on 42 genes involved in breast cancer, including 23 important target genes implicated in the pathophysiology of breast cancer. Pathway analysis using the KEGG database showed a close association between EBE and crucial signaling pathways in breast cancer, including P53 signaling pathway, MAPK signaling pathway, PI3K-Akt signaling pathway, apoptosis and cell cycle. In vitro experiments demonstrated that EBE exhibited moderate anti-cancer activity. Furthermore, EBE demonstrated significant potential in inducing apoptosis in breast cancer cells, with a percentage of apoptotic cells reaching 93.6%. Additionally, EBE was observed to disrupt the cell cycle, leading to a significant increase in the sub G1 and S phases, and a significant decrease in the G2-M and G1 phases.
EBE has the potential to be an anti-cancer agent through various mechanisms, including apoptosis induction and cell cycle inhibition in breast cancer cells. These findings provide new insights into the potential of EBE as an alternative treatment for breast cancer.
本研究旨在通过网络药理学方法和体外验证评估鳞叶薯蓣（EBE）中的植物化学物质在诱导乳腺癌细胞凋亡和抑制细胞周期方面的潜力和机制。
本研究采用文献综述的方法来确定活性抗癌化合物，并利用网络药理学方法来预测 EBE 化合物在乳腺癌中的作用机制。此外，采用 MTT 法评估 EBE 对 T47D 细胞毒性的影响，采用流式细胞术法确定 EBE 对细胞凋亡和细胞周期的影响。
网络药理学分析表明，EBE 对 42 个参与乳腺癌的基因产生影响，包括与乳腺癌病理生理学相关的 23 个重要靶基因。使用 KEGG 数据库进行的通路分析表明，EBE 与乳腺癌中关键的信号通路密切相关，包括 P53 信号通路、MAPK 信号通路、PI3K-Akt 信号通路、凋亡和细胞周期。体外实验表明，EBE 具有中等抗癌活性。此外，EBE 在诱导乳腺癌细胞凋亡方面表现出显著的潜力，凋亡细胞的百分比达到 93.6%。此外，EBE 被观察到破坏细胞周期，导致 S 期和 G1 期显著增加，G2-M 期和 G1 期显著减少。
EBE 通过诱导乳腺癌细胞凋亡和抑制细胞周期等多种机制具有抗癌潜力。这些发现为 EBE 作为乳腺癌替代治疗的潜力提供了新的见解。
