Singh Nalini, Chatterjee Aditi, Chanu Wahengbam Kabita, Vaishalli Pradeep Mini, Singh Chingakham Brajakishor, Nagaraj Viswanathan Arun
Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India.
School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, 751024, Odisha, India.
J Tradit Complement Med. 2023 May 31;13(6):550-560. doi: 10.1016/j.jtcme.2023.05.004. eCollection 2023 Nov.
Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phytochemical analyses of MJ Roem aqueous methanolic leaf extract (MLE).
antiplasmodial studies in () 3D7 and Cam3.I strains were performed by [H]-hypoxanthine uptake assays. cytotoxicity in HeLa and HEK293T cell lines was evaluated using MTT assays. Hemolysis assay was performed using RBCs. Phytochemical analysis by GC-MS and antioxidant studies by DPPH and ABTS assays were performed. antimalarial studies in -infected mice were carried out using Rane's test and Peters' 4-day test.
MLE showed significant antioxidant activity and had phytochemicals reported for antimalarial activity. studies showed prominent antiplasmodial activity against 3D7 strain (IC ∼22 μg/ml) and Cam3. Istrain (IC value ∼43 μg/ml). cytotoxicity studies, hemolytic assays, and acute toxicity studies further suggested that MLE is nontoxic. antimalarial studies using Rane's test showed a significant decrease in parasitemia by ∼70% at 1200 mg/kg doses and delayed the mortality of mice by ∼10-14 days. Peters' 4-day test also showed a similar pattern. The present study demonstrated the antimalarial potential of MLE. These findings deliver a platform for further studies to identify the active components of MLE and discover new antimalarials.
疟疾是一个全球性的健康问题，会导致大量发病和死亡。筛选各种传统上重要的药用植物是发现新型抗疟药物的关键来源。我们评估了MJ Roem水甲醇叶提取物（MLE）的抗疟和抗氧化活性，并进行了详细的植物化学分析。
通过[H] - 次黄嘌呤摄取试验对（）3D7和Cam3.I菌株进行抗疟原虫研究。使用MTT试验评估HeLa和HEK293T细胞系中的细胞毒性。使用红细胞进行溶血试验。通过气相色谱 - 质谱联用进行植物化学分析，并通过DPPH和ABTS试验进行抗氧化研究。使用Rane试验和彼得斯4天试验对感染疟疾的小鼠进行抗疟研究。
MLE显示出显著的抗氧化活性，并且含有据报道具有抗疟活性的植物化学物质。研究表明，MLE对3D7菌株（IC约为22μg/ml）和Cam3.I菌株（IC值约为43μg/ml）具有显著的抗疟原虫活性。细胞毒性研究、溶血试验和急性毒性研究进一步表明MLE无毒。使用Rane试验进行的抗疟研究表明，在1200mg/kg剂量下，疟原虫血症显著降低约70%，并使小鼠的死亡延迟约10 - 14天。彼得斯4天试验也显示出类似的模式。本研究证明了MLE的抗疟潜力。这些发现为进一步研究确定MLE的活性成分和发现新型抗疟药物提供了一个平台。
