Antimalarial activity of MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties.

BACKGROUND AND AIM

Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phytochemical analyses of MJ Roem aqueous methanolic leaf extract (MLE).

EXPERIMENTAL PROCEDURES

antiplasmodial studies in () 3D7 and Cam3.I strains were performed by [H]-hypoxanthine uptake assays. cytotoxicity in HeLa and HEK293T cell lines was evaluated using MTT assays. Hemolysis assay was performed using RBCs. Phytochemical analysis by GC-MS and antioxidant studies by DPPH and ABTS assays were performed. antimalarial studies in -infected mice were carried out using Rane's test and Peters' 4-day test.

RESULTS AND CONCLUSIONS

MLE showed significant antioxidant activity and had phytochemicals reported for antimalarial activity. studies showed prominent antiplasmodial activity against 3D7 strain (IC ∼22 μg/ml) and Cam3. Istrain (IC value ∼43 μg/ml). cytotoxicity studies, hemolytic assays, and acute toxicity studies further suggested that MLE is nontoxic. antimalarial studies using Rane's test showed a significant decrease in parasitemia by ∼70% at 1200 mg/kg doses and delayed the mortality of mice by ∼10-14 days. Peters' 4-day test also showed a similar pattern. The present study demonstrated the antimalarial potential of MLE. These findings deliver a platform for further studies to identify the active components of MLE and discover new antimalarials.