Kimelman D, Kirschner M, Scherson T
Cell. 1987 Feb 13;48(3):399-407. doi: 10.1016/0092-8674(87)90191-7.
The midblastula transition (MBT) in Xenopus can be initiated prematurely by blocking the fundamental cell-cycle oscillator with cycloheximide, in which case motility and transcription are quickly initiated. Using various inhibitors of specific events of the cell cycle that do not inhibit the autonomous oscillator, we have shown that transcription is activated when DNA synthesis is interrupted and motility is activated when cell cleavage is inhibited. Furthermore, very low levels of transcription are found to occur before the MBT. These results demonstrate that the pre-MBT egg is fully competent for transcription and motility and suggest that different features of the rapid early cell cycle normally suppress these events.
非洲爪蟾的中囊胚转换（MBT）可以通过用环己酰亚胺阻断基本的细胞周期振荡器而提前启动，在这种情况下，运动性和转录会迅速启动。使用各种不抑制自主振荡器的细胞周期特定事件的抑制剂，我们已经表明，当DNA合成被中断时转录被激活，当细胞分裂被抑制时运动性被激活。此外，发现在中囊胚转换之前就会发生非常低水平的转录。这些结果表明，中囊胚转换前的卵完全具备转录和运动的能力，并表明快速早期细胞周期的不同特征通常会抑制这些事件。
