Marini S, Guadagni F, Bonmassar E, Potenza P, Giuliani A
Cell Immunol. 1986 Oct 1;102(1):113-25. doi: 10.1016/0008-8749(86)90330-8.
Murine lymphoma cells (YAC-1), induced by Moloney leukemia virus, nontreated (YAC) or pretreated in vitro with interferon (YAC-IF), were tested for their susceptibility to natural killer (NK)-mediated cytolysis. In line with previous reports YAC-IF were less susceptible to NK lysis than YAC cells. In cold competition assay, YAC-IF inhibited cytotoxicity to a lesser extent than YAC lymphoma when labeled target YAC cells were used. However, when radioactive YAC-IF cells were used as targets, cold competition attained with both YAC and YAC-IF was essentially the same. Furthermore, effector splenocytes, depleted of NK effector cells through immunoabsorption on YAC monolayer, were inactive against both YAC and YAC-IF targets. On the other hand, effector lymphocytes, absorbed on YAC-IF monolayer, retained NK activity against YAC cells but not against YAC-IF targets. These results are compatible with the hypothesis that interferon (IF) modulates negatively a subset of "interferon-susceptible" (IFS) NK target structure(s) (TS) of YAC cells, which would then express membrane determinants not functionally present on YAC-IF cells. On the other hand YAC and YAC-IF cells share "interferon-resistant" (IFR) TS not affected by pretreatment with IF. In order to test whether IFS X TS and IFR X TS are present on the same cell or clonally distributed, YAC cells were cloned and tested for NK susceptibility following IF pretreatment. The results did not support the hypothesis of a clonal distribution of both IFS X TS and IFR X TS since IF pretreatment of all clones, obtained by limiting dilution, resulted in a net impairment of target susceptibility to NK effector cells.
由莫洛尼白血病病毒诱导产生的鼠淋巴瘤细胞（YAC-1），未处理组（YAC）或体外经干扰素预处理组（YAC-IF），检测其对自然杀伤（NK）介导的细胞溶解的敏感性。与先前报道一致，YAC-IF比YAC细胞对NK裂解的敏感性更低。在冷竞争试验中，当使用标记的靶YAC细胞时，YAC-IF对细胞毒性的抑制程度低于YAC淋巴瘤。然而，当使用放射性YAC-IF细胞作为靶细胞时，YAC和YAC-IF的冷竞争基本相同。此外，通过在YAC单层上进行免疫吸附去除NK效应细胞的效应脾细胞，对YAC和YAC-IF靶细胞均无活性。另一方面，吸附在YAC-IF单层上的效应淋巴细胞保留了对YAC细胞的NK活性，但对YAC-IF靶细胞无活性。这些结果与以下假设相符：干扰素（IF）对YAC细胞的“干扰素敏感”（IFS）NK靶结构（TS）的一个亚群进行负调节，然后这些结构会表达YAC-IF细胞上不存在功能的膜决定簇。另一方面，YAC和YAC-IF细胞共享不受IF预处理影响的“干扰素抗性”（IFR）TS。为了测试IFS×TS和IFR×TS是存在于同一细胞上还是克隆分布，对YAC细胞进行克隆，并在IF预处理后检测其对NK的敏感性。结果不支持IFS×TS和IFR×TS均克隆分布的假设，因为通过有限稀释获得的所有克隆经IF预处理后，靶细胞对NK效应细胞的敏感性出现净损伤。
