AlSaleh Abdullah, Shahid Mohammad, Farid Eman, Bindayna Khalid M
Microbiology, Immunology and Infectious Diseases, Arabian Gulf University, Manama, BHR.
Pathology/Immunology, Salmaniya Medical Complex, Ministry of Health, College of Medicine, Arabian Gulf University, Manama, BHR.
Cureus. 2023 Oct 24;15(10):e47588. doi: 10.7759/cureus.47588. eCollection 2023 Oct.
Background Panton-Valentine leukocidin (PVL) is one of the most important determinants of virulence in It is associated with a propensity for complicating skin and soft tissue infections and necrotizing pneumonia. This study aims to quantitively examine the effect of ascorbic acid and nicotinamide on PVL production in the reference strain USA300. Methodology Sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantitively measure the production of PVL via the commercial LukS sandwich ELISA kit (IBT Bio-services, MD, USA). Results Incubating USA300 with subinhibitory concentrations of antioxidants resulted in a statistically significant eight-fold reduction in PVL production at 1.25 mg/mL and 30 mg/mL for ascorbic acid and nicotinamide, respectively. Although the mechanism by which antioxidants inhibit PVL production is yet to be elucidated, we suggest that it can be due to interrupting PVL gene expression. Conclusions Ascorbic acid and nicotinamide have the potential to be toxin-suppressing agents that may be effective in supporting the bactericidal effect of antibiotics to improve the outcome of PVL-associated infections; however, further extensive research is required.
背景 潘顿-瓦伦丁杀白细胞素（PVL）是[具体细菌名称未给出]毒力的最重要决定因素之一。它与皮肤和软组织感染及坏死性肺炎的并发症倾向有关。本研究旨在定量检测抗坏血酸和烟酰胺对参考菌株USA300中PVL产生的影响。方法 采用夹心酶联免疫吸附测定（ELISA）法，通过商用LukS夹心ELISA试剂盒（美国马里兰州IBT生物服务公司）定量测定PVL的产生。结果 用亚抑制浓度的抗氧化剂孵育USA300，结果显示，抗坏血酸和烟酰胺分别在1.25 mg/mL和30 mg/mL时，PVL产生量在统计学上显著降低了8倍。尽管抗氧化剂抑制PVL产生的机制尚待阐明，但我们认为这可能是由于干扰了PVL基因表达。结论 抗坏血酸和烟酰胺有可能成为毒素抑制剂，可能有效支持抗生素的杀菌作用，从而改善与PVL相关感染的治疗效果；然而，还需要进一步广泛研究。
