Shahriarirad Reza, Erfani Amirhossein, Nekouei Fatemeh, Seifbehzad Sarvin, Hosseinzadeh Masood, Sarkari Bahador, Tanideh Nader, Koohi-Hosseinabadi Omid, Nassour Nour, Ashkani-Esfahani Soheil
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran (Reza Shahriarirad, Amirhossein Erfani, Fatemeh Nekouei, Sarvin Seifbehzad).
Thoracic and Vascular Surgery Research Center, Shiraz University of Medical Sciences, Shiraz, Iran (Reza Shahriarirad, Amirhossein Erfani).
Ann Gastroenterol. 2023 Nov-Dec;36(6):654-660. doi: 10.20524/aog.2023.0836. Epub 2023 Oct 30.
Inflammation and oxidative activities within the gut play major roles in the pathogenesis of ulcerative colitis (UC). We aimed to determine the effect of , an antioxidant and anti-inflammatory agent, on the colon histological characteristics in acetic acid (AA)-induced UC in rat models.
Thirty-six male rats with AA-induced colitis were divided into 5 groups: no treatment (AA); daily treatment with 300 mg/kg orally (MO) and rectally (MR); and 100 mg/kg mesalamine orally (AO) and rectally (AR). Macroscopic and histopathological evaluation of the colon, along with a biochemical laboratory evaluation, were performed 10 days after UC induction.
All treatment groups demonstrated lower macroscopic grading scores compared to the AA group. After treatment with MO, 42.9% of cases demonstrated no macroscopic changes, while 14.3% demonstrated only mucosal erythema. In the MR group 28.6% of rats had no changes in their mucosal lining and 28.6% had only mucosal erythema. Following histopathological evaluation, the AO group had lower scores regarding the severity of ulcer, inflammation, destruction, crypt abscess, and disorganization compared to the MO group. (P=0.02) The MR group demonstrated lower microscopic scores compared to the MO group, and also lower macroscopic scores compared to the AR group, although not significantly (P>0.05).
Both oral and topical administration of have satisfactory healing properties compared to mesalamine, with topical route having better results. Therefore, further studies are needed to establish the benefit of administration (both orally and topically) within a UC treatment protocol.
肠道内的炎症和氧化活动在溃疡性结肠炎（UC）的发病机制中起主要作用。我们旨在确定一种抗氧化和抗炎剂对醋酸（AA）诱导的大鼠UC模型结肠组织学特征的影响。
将36只AA诱导的结肠炎雄性大鼠分为5组：不治疗（AA组）；每日口服（MO）和直肠给药（MR）300mg/kg；以及口服（AO）和直肠给药（AR）100mg/kg美沙拉嗪。在诱导UC后10天进行结肠的宏观和组织病理学评估以及生化实验室评估。
与AA组相比，所有治疗组的宏观分级评分均较低。MO治疗后，42.9%的病例无宏观变化，而14.3%仅表现为黏膜红斑。在MR组中，28.6%的大鼠黏膜层无变化，28.6%仅表现为黏膜红斑。组织病理学评估后，与MO组相比，AO组在溃疡、炎症、破坏、隐窝脓肿和结构紊乱的严重程度方面得分较低（P = 0.02）。MR组与MO组相比显微镜下得分较低，与AR组相比宏观得分也较低，尽管差异不显著（P>0.05）。
与美沙拉嗪相比，口服和局部应用均具有令人满意的愈合特性，局部给药效果更好。因此，需要进一步研究以确定在UC治疗方案中应用（口服和局部）的益处。
