Jalalipour Maryam, Yegdaneh Afsaneh, Talebi Ardeshir, Minaiyan Mohsen
School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Res Pharm Sci. 2022 Jul 14;17(4):350-359. doi: 10.4103/1735-5362.350236. eCollection 2022 Aug.
Inflammatory bowel disease (IBD) is a chronic and multifactorial disease with unknown etiology and a decisive cure. (sage) which has anti-inflammatory, anti-oxidative, and ulcer healing properties can be useful for the treatment of IBD. Therefore, the effect of ethanolic extract (SOEE) and methanolic partition (SOMP) was investigated on acetic acid-induced ulcerative colitis.
Male Wistar rats (180-220 g) were used. SOEE (30, 60, and 120 mg/kg) and SOMP (50, 100, and 150 mg/kg) were prepared through maceration method. Prepared extracts, dexamethasone (1 mg/kg, i.p.), and mesalamine (100 mg/kg) as reference drugs and normal saline as control were administered by gavage, 2 h before colitis induction and preserved for four further days to animals. The colon tissues were examined for macroscopic and pathologic parameters and myeloperoxidase (MPO) and malondialdehyde (MDA) levels.
FINDINGS/RESULTS: SOEE (60 and 120 mg/kg) and SOMP at all doses alleviated colitis severity and indices both in macroscopic and microscopic views. MDA and MPO activities were also significantly declined in the extracts-treated groups compared to the controls. The lowest dose of SOEE couldn't meaningfully reduce any of the parameters compared to the control group.
Both extracts of exerted anti-colitis effects in rats, though methanolic partition was more effective, especially at the highest dose. It seems S. officinalis could exert protection against oxidative stress and inflammatory mediators in colitis tissue. More experimental and clinical studies are required to explore the exact mechanisms and active ingredients which are involved.
炎症性肠病（IBD）是一种病因不明且难以根治的慢性多因素疾病。具有抗炎、抗氧化和促进溃疡愈合特性的鼠尾草（sage）可能对IBD的治疗有用。因此，研究了乙醇提取物（SOEE）和甲醇分离物（SOMP）对乙酸诱导的溃疡性结肠炎的影响。
使用雄性Wistar大鼠（180 - 220克）。通过浸渍法制备SOEE（30、60和120毫克/千克）和SOMP（50、100和150毫克/千克）。在诱导结肠炎前2小时，通过灌胃给予制备好的提取物、作为参考药物的地塞米松（1毫克/千克，腹腔注射）和美沙拉嗪（100毫克/千克），并将动物再饲养四天。检查结肠组织的宏观和病理参数以及髓过氧化物酶（MPO）和丙二醛（MDA）水平。
SOEE（60和120毫克/千克）以及所有剂量的SOMP在宏观和微观层面均减轻了结肠炎的严重程度和各项指标。与对照组相比，提取物处理组的MDA和MPO活性也显著下降。与对照组相比，最低剂量的SOEE未能显著降低任何参数。
两种提取物在大鼠中均发挥了抗结肠炎作用，尽管甲醇分离物更有效，尤其是在最高剂量时。鼠尾草似乎可以对结肠炎组织中的氧化应激和炎症介质起到保护作用。需要更多的实验和临床研究来探索其中的确切机制和活性成分。
