Lu Lijuan, Zhong Huaqing, Xu Menghua, Jia Ran, Liu Pengcheng, Su Liyun, Cao Lingfeng, Zhu Xunhua, Xu Jin
Department of Clinical Laboratory, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai, China.
Shanghai Institute of Infectious Disease and Biosecurity, Fudan University, Shanghai, China.
Front Microbiol. 2023 Nov 13;14:1265843. doi: 10.3389/fmicb.2023.1265843. eCollection 2023.
Human astrovirus (HAstV) is an important pathogen of acute gastroenteritis (AGE) in children. This study was aimed at investigating the diversity and epidemiology of classic and novel HAstV in outpatient children aged 0-16 years old with AGE in Shanghai.
From May 2020 to December 2022, a total of 1,482 stool samples were collected from children diagnosed as AGE from the Children's Hospital of Fudan University. HAstV was identified using pan-astrovirus consensus primers by Reverse transcription PCR.
During the study period, 3.3% (49/1,482) of specimens were identified as HAstV, with a detection rate of 2.5% (37/1,482) for classic HAstV and 0.8% (12/1,482) for novel HAstV. Among the 12 novel HAstV strains, 11 (91.7%) belonged to the HAstV-MLB and 1 (8.3%) was HAstV-VA. Genotyping revealed six circulating genotypes. Strain HAstV-1 was predominant in the study population with a detection rate of 1.8% (26/1,482) followed by HAstV-MLB1 (0.7%, 10/1,482) and HAstV-4 (0.6%, 9/1,482). Of note, all the HAstV-4 strains detected in this study were close to one astrovirus strain isolated from Bactrian camels with 99.0-100.0% amino acid sequences identity. In this study, HAstV was detected in all age groups with the highest detection rate of HAstV-positive specimens observed in children older than 73 months (5.7%, 12/209).
This study provided useful information and contributed to the molecular epidemiology of both classic and novel HAstV, which were simultaneously characterized and reported for the first time in Shanghai.
人星状病毒（HAstV）是儿童急性胃肠炎（AGE）的重要病原体。本研究旨在调查上海0至16岁门诊AGE患儿中经典和新型HAstV的多样性及流行病学特征。
2020年5月至2022年12月，从复旦大学附属儿科医院诊断为AGE的患儿中收集了共1482份粪便样本。通过逆转录聚合酶链反应使用泛星状病毒通用引物鉴定HAstV。
在研究期间，3.3%（49/1482）的标本被鉴定为HAstV，经典HAstV的检出率为2.5%（37/1482），新型HAstV的检出率为0.8%（12/1482）。在12株新型HAstV毒株中，11株（91.7%）属于HAstV-MLB，1株（8.3%）为HAstV-VA。基因分型显示有六种流行基因型。HAstV-1毒株在研究人群中占主导地位，检出率为1.8%（26/1482），其次是HAstV-MLB1（0.7%，10/1482）和HAstV-4（0.6%，9/1482）。值得注意的是，本研究中检测到的所有HAstV-4毒株均与一株从双峰驼分离的星状病毒毒株相近，氨基酸序列同一性为99.0 - 100.0%。在本研究中，所有年龄组均检测到HAstV，73个月以上儿童中HAstV阳性标本的检出率最高（5.7%，12/209）。
本研究提供了有用信息，有助于经典和新型HAstV的分子流行病学研究，这两种病毒在上海首次同时得到鉴定和报道。
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