Department of Oral Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, New Delhi, India.
Department of Oral Pathology and Microbiology, Maulana Azad Institute of Dental Sciences, New Delhi, India.
Pathol Res Pract. 2024 Jan;253:154954. doi: 10.1016/j.prp.2023.154954. Epub 2023 Nov 21.
microRNA(miRNA)-196a and miRNA-196b expression has been found to be dysregulated and involved in tumorigenesis and tumor progression in array of cancers through different targets. The role of these miRNAs together in clinical application is not always consistent and, its prognostic value in oral squamous cell carcinoma (OSCC) is still elusive. This study was performed to investigate the correlation of these miRNAs expression with histological grades of OSCC according to Bryne's histological grading system, to predict prognosis and to evaluate their relationship with clinico-pathological data.
Real-time quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was done to evaluate the expression levels of miRNA-196a and miRNA-196b in 75 pairs of OSCC tissue matched with adjacent normal mucosa, used as a control.
miRNA-196a and 196b expression in OSCC was significantly higher than that in corresponding adjacent normal tissues (p > 0.001). Also, a significant differential correlation was found in between the expression levels of these two miRNAs (Pearson correlation test r = 0.676, p-value<0.0001). The increased expression of these miRNAs was more frequently observed in OSCC tissues with advanced clinical and pathological TNM stages (IVa and IVb, pIVb respectively, p-value<0.0001). Significant correlation was found between miRNA-196a upregulation and moderate prognostic score (p-value<0.0001) in comparison with good and poor prognostic score of histological grades of OSCC. Sensitivity and specificity for miRNA-196a were 95 % and 85 %, respectively (AUC = 1, 95 % CI = 0.617-0.850; p 0.001), while for miRNA-196b were 94 % and 86 %, respectively (AUC = 0.808, 95 % CI = 0.701-0.916; p0.0001).
These findings suggest that the increased expression of miRNA-196a and 196b may play an important role in tumor progression in OSCC. miRNA-196a might be a useful marker for predicting the clinical outcome of OSCC, especially for advanced stages. In conclusion, our data demonstrate for the first time that these miRNAs may serve as a potent prognostic marker for tumor progression. We further highlight miRNA-196a and miRNA-196b as a promising predictor of prognostic assessment in OSCC.
通过不同的靶点，miRNA-196a 和 miRNA-196b 的表达失调已被发现与多种癌症的肿瘤发生和肿瘤进展有关。这些 miRNA 一起在临床应用中的作用并不总是一致的，其在口腔鳞状细胞癌 (OSCC) 中的预后价值仍不清楚。本研究旨在根据 Bryne 的组织学分级系统，研究这些 miRNA 表达与 OSCC 组织学分级的相关性，预测预后，并评估其与临床病理数据的关系。
采用实时定量逆转录聚合酶链反应 (qRT-PCR) 检测 75 对 OSCC 组织及其配对的相邻正常黏膜中 miRNA-196a 和 miRNA-196b 的表达水平，作为对照。
OSCC 中 miRNA-196a 和 196b 的表达明显高于相应的相邻正常组织 (p>0.001)。此外，这两种 miRNA 的表达水平之间存在显著的差异相关关系 (Pearson 相关检验 r=0.676，p 值<0.0001)。这些 miRNA 的表达增加在临床和病理 TNM 分期较晚的 OSCC 组织中更为常见 (IVa 和 IVb，分别为 pIVb，p 值<0.0001)。与 OSCC 组织学分级的良好和不良预后评分相比，miRNA-196a 的上调与中等预后评分显著相关 (p 值<0.0001)。miRNA-196a 的敏感性和特异性分别为 95%和 85% (AUC=1，95%CI=0.617-0.850;p<0.001)，而 miRNA-196b 分别为 94%和 86% (AUC=0.808，95%CI=0.701-0.916;p<0.0001)。
这些发现表明，miRNA-196a 和 196b 的表达增加可能在 OSCC 的肿瘤进展中发挥重要作用。miRNA-196a 可能是预测 OSCC 临床结局的有用标志物，特别是对于晚期患者。总之，我们的数据首次表明，这些 miRNA 可能作为肿瘤进展的有力预后标志物。我们进一步强调 miRNA-196a 和 miRNA-196b 作为 OSCC 预后评估的有前途的预测因子。
