Sun Lanfang, Zhou Junyi, Lai Jieying, Zheng Xue, Zhang Li-Ming
DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
DSAPM Lab and PCFM Lab, School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
Int J Biol Macromol. 2024 Jan;256(Pt 2):128505. doi: 10.1016/j.ijbiomac.2023.128505. Epub 2023 Nov 29.
Developing wound dressings with solid adhesive properties that enable efficient, painless hemostasis and prevent wound infection remain a huge challenge. Herein, the tris(hydroxymethyl) methyl glycine-modified chitosan derivative (CTMG) was prepared and freeze-dried after simply adjusting the concentration of CTMG to obtain the chitosan-based gel sponge with desired multi-hollow structure, special antibacterial and biocompatibility. The adhesion strength on porcine skin was impressive up to 113 KPa, much higher than fibrin glue. It can withstand the pressure that far exceeds blood pressure. CTMG exhibits bacteriostatic abilities as demonstrated in a bacteriostatic assay, and alongside biocompatibility, as shown in cytotoxicity and hemolytic assays. Moreover, CTMG gel sponge showed hemostatic properties in both in vivo and in vitro hemostasis experiments. During an experiment on liver hemorrhage in rats, CTMG gel sponge proved to be more effective in controlling bleeding than other hemostatic sponges available on the market, indicating its promising hemostatic properties. CTMG gel sponge possesses the potential to function as a wound dressing and hemostatic material, making it suitable for various clinical applications.
开发具有固体粘附特性的伤口敷料，以实现高效、无痛止血并预防伤口感染，仍然是一项巨大的挑战。在此，制备了三（羟甲基）甲基甘氨酸修饰的壳聚糖衍生物（CTMG），并在简单调整CTMG浓度后进行冷冻干燥，以获得具有所需多中空结构、特殊抗菌性和生物相容性的壳聚糖基凝胶海绵。其在猪皮上的粘附强度高达113 KPa，令人印象深刻，远高于纤维蛋白胶。它能够承受远远超过血压的压力。抑菌试验表明CTMG具有抑菌能力，细胞毒性和溶血试验则显示其具有生物相容性。此外，CTMG凝胶海绵在体内和体外止血实验中均表现出止血特性。在大鼠肝出血实验中，CTMG凝胶海绵在控制出血方面比市场上现有的其他止血海绵更有效，表明其具有良好的止血性能。CTMG凝胶海绵具有作为伤口敷料和止血材料的潜力，适用于各种临床应用。
