Li Meng, Zhang Zhiyi, Liang Yongping, He Jiahui, Guo Baolin
Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710049, China.
ACS Appl Mater Interfaces. 2020 Aug 12;12(32):35856-35872. doi: 10.1021/acsami.0c08285. Epub 2020 Jul 29.
Cryogels with tissue adhesion have great potential as wound dressings for rapid hemostasis for uncontrollable nonpressing surface hemorrhage and wound healing, but their use has not been reported previously. Herein, we designed a series of antibacterial and antioxidant tissue-adhesive cryogels based on quaternized chitosan (QCS) and polydopamine (PDA). These cryogels had good blood cell and platelet adhesion, enrichment, and activation properties for rapid nonpressing surface hemostasis and wound healing. The cryogels exhibited outstanding mechanical strength and easy removability, antioxidant activity, and NIR photothermal-enhanced antibacterial performance. The cryogels showed much better hemostasis than gauze and gelatin sponge in a standardized strip rat liver injury model, a standardized circular rabbit liver section model, and a pig skin laceration model. Furthermore, the excellent hemostatic performance of the QCS/PDA2.0 cryogel (containing 20 mg/mL QCS and 2.0 mg/mL PDA) for coagulopathic hemorrhages was confirmed in a standardized coagulation disorder rabbit circular liver section model. In addition, the QCS/PDA2.0 cryogel promoted rapid hemostasis in a deep noncompressible wound and a much better wound healing effect than a chitosan sponge and Tegaderm film in a full-thickness skin defect model. Overall, these multifunctional tissue-adhesive cryogels with excellent hemostatic performance and enhanced wound healing properties are suitable candidates for tissue-adhesive hemostat and wound healing dressings.
具有组织粘附性的冷冻凝胶作为用于不可控非压迫性表面出血快速止血和伤口愈合的伤口敷料具有巨大潜力，但此前尚未见相关报道。在此，我们基于季铵化壳聚糖（QCS）和聚多巴胺（PDA）设计了一系列抗菌和抗氧化的组织粘附性冷冻凝胶。这些冷冻凝胶具有良好的血细胞和血小板粘附、富集及活化特性，可实现快速的非压迫性表面止血和伤口愈合。该冷冻凝胶具有出色的机械强度、易于去除性、抗氧化活性以及近红外光热增强抗菌性能。在标准化的条形大鼠肝损伤模型、标准化的圆形兔肝切片模型和猪皮肤裂伤模型中，冷冻凝胶的止血效果比纱布和明胶海绵好得多。此外，在标准化的凝血障碍兔圆形肝切片模型中，证实了QCS/PDA2.0冷冻凝胶（含有20 mg/mL QCS和2.0 mg/mL PDA）对凝血功能障碍性出血具有优异的止血性能。另外，在全层皮肤缺损模型中，QCS/PDA2.0冷冻凝胶在深部不可压迫伤口中促进了快速止血，且伤口愈合效果比壳聚糖海绵和德湿可薄膜好得多。总体而言，这些具有优异止血性能和增强伤口愈合特性的多功能组织粘附性冷冻凝胶适合作为组织粘附性止血剂和伤口愈合敷料。
