Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China.
Department of Stomatology, Wuxi Children's Hospital, Wuxi 214023, PR China.
Colloids Surf B Biointerfaces. 2022 Oct;218:112770. doi: 10.1016/j.colsurfb.2022.112770. Epub 2022 Aug 12.
Uncontrolled bleeding leads to a higher fatality rate in the situation of surgery, traffic accidents and warfare. Traditional hemostatic materials such as bandages are not ideal for uncontrolled or incompressible bleeding. Therefore, it is of great significance to develop a new medical biomaterial with excellent rapid hemostatic effect. Keratin is a natural, biocompatible and biodegradable protein which contains amino acid sequences that induce cell adhesion. As a potential biomedical material, keratin has been developed and paid attention in tissue engineering fields such as promoting wound healing and nerve repair. Herein, a keratin/chitosan (K/C) sponge was prepared to achieve rapid hemostasis. The characterizations of K/C sponge were investigated, including SEM, TGA, liquid absorption and porosity, showing that the high porosity up to 90.12 ± 2.17 % resulted in an excellent blood absorption. The cytotoxicity test and implantation experiment proved that the K/C sponge was biocompatible and biodegradable. Moreover, the prepared K/C sponge showed better hemostatic performance than chitosan sponge (CS) and the commercially available gelatin sponge in both rat tail amputation and liver trauma bleeding models. Further experiments showed that K/C sponge plays a hemostatic role through the endogenous coagulation pathway, thus shortening the activated partial thromboplastin time (APTT) effectively. Therefore, this study provided a K/C sponge which can be served as a promising biomedical hemostatic material.
在手术、交通事故和战争等情况下，无法控制的出血会导致更高的死亡率。传统的止血材料，如绷带，对于无法控制或不可压缩的出血并不理想。因此，开发一种具有优异快速止血效果的新型医用生物材料具有重要意义。角蛋白是一种天然的、生物相容的和可生物降解的蛋白质，它含有诱导细胞黏附的氨基酸序列。角蛋白作为一种有潜力的生物医学材料，已经在组织工程领域得到了开发和关注，例如促进伤口愈合和神经修复。在此，我们制备了一种角蛋白/壳聚糖（K/C）海绵以实现快速止血。对 K/C 海绵的特性进行了研究，包括 SEM、TGA、液体吸收和孔隙率，结果表明其高达 90.12±2.17%的高孔隙率导致了优异的血液吸收能力。细胞毒性试验和植入实验证明了 K/C 海绵具有良好的生物相容性和可降解性。此外，与壳聚糖海绵（CS）和市售明胶海绵相比，所制备的 K/C 海绵在大鼠尾切断和肝创伤出血模型中表现出更好的止血性能。进一步的实验表明，K/C 海绵通过内源性凝血途径发挥止血作用，从而有效缩短活化部分凝血活酶时间（APTT）。因此，本研究提供了一种 K/C 海绵，可作为一种有前途的生物医学止血材料。
