Belluati Andrea, Happel Dominic, Erbe Malte, Kirchner Nicole, Szelwicka Anna, Bloch Adrian, Berner Valeria, Christmann Andreas, Hertel Brigitte, Pardehkhorram Raheleh, Reyhani Amin, Kolmar Harald, Bruns Nico
Department of Chemistry, Technical University of Darmstadt, Peter-Grünberg-Straße 4, 64287 Darmstadt, Germany.
Centre for Synthetic Biology, Technical University of Darmstadt, Merckstraße 25, 64283 Darmstadt, Germany.
Nanoscale. 2023 Dec 14;15(48):19486-19492. doi: 10.1039/d3nr04008a.
Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.
通过创新性地使用表面展示的辣根过氧化物酶，这项工作探索了生物RAFT聚合和生物ATRP的酶促催化作用，以促进细胞表面的聚合物合成，其中生物ATRP的表现优于生物RAFT聚合。用合成聚合物对活酵母细胞进行表面修饰，可使酵母表型发生显著变化，包括生长曲线、聚集特性，以及非天然酶与细胞表面可点击聚合物的共轭，为生物正交细胞表面工程开辟了新途径。
