Danielson Alex P, Bailey-Van Kuren Dylan, Lucius Melissa E, Makaroff Katherine, Williams Cameron, Page Richard C, Berberich Jason A, Konkolewicz Dominik
Department of Chemistry and Biochemistry, Miami University, 651 E High St, Oxford, OH, 45056, USA.
Department of Chemical, Paper and Biomedical Engineering, Miami University, 650 E High St, Oxford, OH, 45056, USA.
Macromol Rapid Commun. 2016 Feb;37(4):362-7. doi: 10.1002/marc.201500633. Epub 2016 Jan 8.
Enzymatic catalysis and control over macromolecular architectures from reversible addition-fragmentation chain transfer polymerization (RAFT) are combined to give a new method of making polymers. Horseradish peroxidase (HRP) is used to catalytically generate radicals using hydrogen peroxide and acetylacetone as a mediator. RAFT is used to control the polymer structure. HRP catalyzed RAFT polymerization gives acrylate and acrylamide polymers with relatively narrow molecular weight distributions. The polymerization is rapid, typically exceeding 90% monomer conversion in 30 min. Complex macromolecular architectures including a block copolymer and a protein-polymer conjugate are synthesized using HRP to catalytically initiate RAFT polymerization.
将酶催化与可逆加成-断裂链转移聚合（RAFT）对大分子结构的控制相结合，得到了一种制备聚合物的新方法。使用辣根过氧化物酶（HRP），以过氧化氢和乙酰丙酮作为介质催化产生自由基。利用RAFT控制聚合物结构。HRP催化的RAFT聚合得到分子量分布相对较窄的丙烯酸酯和丙烯酰胺聚合物。聚合反应迅速，通常在30分钟内单体转化率超过90%。使用HRP催化引发RAFT聚合，合成了包括嵌段共聚物和蛋白质-聚合物共轭物在内的复杂大分子结构。
