Yao Shaobo, Gao Zhuyu, Fang Wenhua, Fu Ying, Xue Qianqian, Lai Tianmin, Shangguan Huangcheng, Sun Weiwei, Lin Yuanxiang, Lin Fuxin, Kang Dezhi
From the Departments of Department of Neurosurgery, Department of Nuclear Medicine, Neurosurgery Research Institute.
Department of Neurology, Institute of Neurology, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian.
Clin Nucl Med. 2024 Jan 1;49(1):56-65. doi: 10.1097/RLU.0000000000004948. Epub 2023 Nov 29.
Our aims were to investigate the presence of choroid plexus (CP) inflammation in chronic-phase intracerebral hemorrhage (ICH) patients and to characterize any inflammatory cells in the CP.
An in vivo 18 F-DPA714 PET study was undertaken in 22 chronic-phase ICH patients who were admitted to the First Affiliated Hospital of Fujian Medical University or Tianjin Medical University General Hospital from April 2017 to June 2020. Ten control participants with nonhemorrhagic central nervous system diseases were included. Choroid plexus 18 F-DPA714 uptake was calculated as the average SUVR. To aid the interpretation of the 18 F-DPA714 uptake results at the CP level, Cy5-DPA714 in vivo imaging and immunofluorescence staining were used to show the presence of CP inflammation in an ICH mouse model during the chronic phase (14 weeks after ICH). Then immunofluorescence staining against translocator protein and other specific biomarkers was used to characterize the cells present in the inflamed CP of ICH mice in the chronic phase.
PET imaging showed that CP DPA714 SUVRs in chronic-phase ICH patients were higher than in controls (mean CP SUVR ± SD; ICH group: 1.05 ± 0.35; control group: 0.81 ± 0.21; P = 0.006). Immunofluorescence staining of the CP in ICH model mice identified a population of CD45 + immune cells, peripheral monocyte-derived CD14 + cells, CD68 + phagocytes, and CD11b + resident microglia/macrophages expressing translocator protein, possibly contributing to the increased 18 F-DPA714 uptake.
Our study shows that CP DPA714 uptake in chronic-phase ICH patients was higher than that of participants with nonhemorrhagic central nervous system diseases, which means that CP inflammation is still active in chronic-phase ICH patients.
我们的目的是研究慢性期脑出血（ICH）患者脉络丛（CP）炎症的存在情况，并对CP中的任何炎症细胞进行特征描述。
2017年4月至2020年6月期间，对入住福建医科大学附属第一医院或天津医科大学总医院的22例慢性期ICH患者进行了一项体内18F-DPA714 PET研究。纳入了10名患有非出血性中枢神经系统疾病的对照参与者。脉络丛18F-DPA714摄取量以平均标准化摄取值（SUVR）计算。为了辅助解释CP水平的18F-DPA714摄取结果成像结果，在慢性期（ICH后14周）的ICH小鼠模型中，使用Cy5-DPA714体内成像和免疫荧光染色来显示CP炎症的存在。然后，针对转位蛋白和其他特定生物标志物进行免疫荧光染色，以对慢性期ICH小鼠炎症性CP中存在的细胞进行特征描述。
PET成像显示，慢性期ICH患者的CP DPA714 SUVR高于对照组（平均CP SUVR±标准差；ICH组：1.05±0.35；对照组：0.81±0.21；P = 0.006）。ICH模型小鼠CP的免疫荧光染色鉴定出一群表达转位蛋白的CD45 +免疫细胞、外周单核细胞衍生的CD14 +细胞、CD68 +吞噬细胞和CD11b +常驻小胶质细胞/巨噬细胞，这可能导致18F-DPA714摄取增加。
我们的研究表明，慢性期ICH患者的CP DPA714摄取高于非出血性中枢神经系统疾病参与者，这意味着CP炎症在慢性期ICH患者中仍然活跃。
