College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China.
Meixian Aquaculture Farm of Shitouhe Reservoir Administration, Xianyang 712000, Shaanxi, China.
J Agric Food Chem. 2023 Dec 20;71(50):20118-20130. doi: 10.1021/acs.jafc.3c06768. Epub 2023 Dec 7.
In this study, the coding region of rainbow trout was cloned and sequenced to synthesize a recombinant protein (rFGF21) and investigate its potential role in improving glycolipid metabolism. Acute injection of rFGF21 into rainbow trout effectively reduced serum glucose levels. To investigate the effect of rFGF21 on high-carbohydrate diet (HCD)-induced metabolic disorders in rainbow trout, a 31-day feeding experiment was conducted. At the end of the third week, fish were injected with either PBS or rFGF21. The results showed that the final body weight (FBW) significantly increased in rainbow trout on an HCD ( < 0.05), but there were potential risks including disturbances in glycolipid metabolism and increased inflammatory responses. However, these effects were altered by rFGF21 treatment. In addition, rFGF21 promotes glucose uptake by increasing the phosphorylation levels of AKT (protein kinase B) and GSK3β (glycogen synthase kinase 3β), increasing hepatic glycogen, thereby lowering serum glucose. Notably, the rFGF21 did not exacerbate the inflammatory response but downregulated the expression of inflammatory factors. Interestingly, the activation of autophagy and the AMPK pathway may contribute to the positive effect of rFGF21, where rFGF21 injection significantly increased the levels of LC3I/II protein and phosphorylate AMPKα ( < 0.05).
在这项研究中，克隆并测序了虹鳟鱼的编码区，以合成重组蛋白（rFGF21），并研究其在改善糖脂代谢方面的潜在作用。急性注射 rFGF21 可有效降低虹鳟鱼的血清葡萄糖水平。为了研究 rFGF21 对虹鳟鱼高碳水化合物饮食（HCD）诱导的代谢紊乱的影响，进行了为期 31 天的喂养实验。在第三周结束时，用 PBS 或 rFGF21 注射鱼。结果表明，HCD 组虹鳟鱼的终体重（FBW）显著增加（<0.05），但存在糖脂代谢紊乱和炎症反应增强等潜在风险。然而，rFGF21 处理改变了这些影响。此外，rFGF21 通过增加 AKT（蛋白激酶 B）和 GSK3β（糖原合酶激酶 3β）的磷酸化水平来促进葡萄糖摄取，增加肝糖原，从而降低血清葡萄糖。值得注意的是，rFGF21 没有加剧炎症反应，而是下调了炎症因子的表达。有趣的是，自噬和 AMPK 途径的激活可能是 rFGF21 产生积极作用的原因，rFGF21 注射显著增加了 LC3I/II 蛋白和磷酸化 AMPKα的水平（<0.05）。
