Retention behavior of carbohydrates on metal loaded chelating stationary phase under conditions of hydrophilic interaction liquid chromatography.

The chromatographic retention of carbohydrates on chelating stationary phase loaded with different metal ions was studied under conditions of hydrophilic interaction chromatography (HILIC). The chelating stationary phases represented silica microparticles with immobilized 2-hydroxyethyliminodiacetic acid (HEIDA) groups in loose form and saturated with Ca, Pb, and Laform. The role of loaded metal ion, the acetonitrile and methanol content in the mobile phase, buffer pH and column temperature on the retention of l-(+)-arabinose, d-(+)-maltose, l-(+)-rhamnose, d-(+)-lactose, d-(+)-xylose, glucose, fructose, sucrose, mannose, maltotriose and d-(+) raffinose was studied. The investigation was mainly focused on possible contribution of the complexation in the stationary phase on retention of carbohydrates as well as on effect of the presence metal ion in HEIDA-silica on resulting HILIC behavior of. It is shown that adsorbents with immobilized metal complexes have a good potential for the separation of organic ligands under HILIC mode.