Halliday Mia R, Miller Samantha L, Gale Christopher D, Deckard Jenna R, Gourley Bridget L, Levinger Nancy E
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, United States.
Department of Chemistry and Biochemistry, DePauw University, Greencastle, Indiana 46135-0037, United States.
Langmuir. 2024 Oct 8;40(40):20918-20926. doi: 10.1021/acs.langmuir.4c01826. Epub 2024 Sep 22.
Although all hexose sugars share the same chemical formula, CHO, subtle differences in their stereochemical structures lead to their various biological roles. Due to their prominent role in metabolism, hexose sugars are commonly found in nanoconfined environments. The complexity of authentic nanoconfined biological environments makes it challenging to study how confinement affects their behavior. Here, we present a study using a common model system, AOT reverse micelles, to study hexose sugars in nanoconfinement. We examine how reverse micelles affect the hexoses, how the hexoses affect reverse micelle formation, and the differences between specific hexoses: glucose, mannose, and galactose. We find that addition of glucose, mannose or galactose to reverse micelles that already contain water leaves their size smaller or nearly unchanged. Introducing aqueous hexose solution yields reverse micelles smaller than those prepared with the same volume of water. We use H NMR to show how the nanoconfined environment impacts hexose sugars' anomeric ratios. Nanoconfined mannose and galactose display smaller changes in their anomeric ratios compared to glucose. These conclusions may provide insights about the biological roles of each hexose when studied under a more authentic nanoconfined system.
尽管所有己糖都具有相同的化学式C₆H₁₂O₆，但它们立体化学结构上的细微差异导致了它们具有不同的生物学功能。由于己糖在新陈代谢中起着重要作用，因此它们通常存在于纳米受限环境中。真实的纳米受限生物环境的复杂性使得研究受限如何影响它们的行为具有挑战性。在此，我们展示了一项使用常见模型系统——AOT反胶束来研究纳米受限环境中的己糖的研究。我们研究了反胶束如何影响己糖，己糖如何影响反胶束的形成，以及特定己糖（葡萄糖、甘露糖和半乳糖）之间的差异。我们发现，向已经含有水的反胶束中添加葡萄糖、甘露糖或半乳糖会使它们的尺寸变小或几乎不变。引入己糖水溶液会产生比用相同体积的水制备的反胶束更小的反胶束。我们使用¹H NMR来展示纳米受限环境如何影响己糖的异头物比例。与葡萄糖相比，纳米受限的甘露糖和半乳糖的异头物比例变化较小。这些结论可能为在更真实的纳米受限系统中研究时每种己糖的生物学功能提供见解。
