氦气冷态大气压等离子体导致细胞形态和生化改变。
Helium Cold Atmospheric Plasma Causes Morphological and Biochemical Alterations in Cells.
机构信息
Oral Biopathology Graduate Program, Department of Environment Engineering, São José dos Campos Institute of Science & Technology, São Paulo State University (UNESP), São José dos Campos 12247-016, SP, Brazil.
Department of Materials and Technology, Guaratinguetá Faculty of Engineering and Sciences, São Paulo State University (UNESP), Guaratinguetá 12516-410, SP, Brazil.
出版信息
Molecules. 2023 Dec 3;28(23):7919. doi: 10.3390/molecules28237919.
(1) Background: Previous studies reported the promising inhibitory effect of cold atmospheric plasma (CAP) on . However, the exact mechanisms of CAP's action on the fungal cell are still poorly understood. This study aims to elucidate the CAP effect on cell wall, by evaluating the alterations on its structure and biochemical composition; (2) Methods: cells treated with Helium-CAP were analyzed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in order to detect morphological, topographic and biochemical changes in the fungal cell wall. Cells treated with caspofungin were also analyzed for comparative purposes; (3) Results: Expressive morphological and topographic changes, such as increased roughness and shape modification, were observed in the cells after CAP exposure. The alterations detected were similar to those observed after the treatment with caspofungin. The main biochemical changes occurred in polysaccharides content, and an overall decrease in glucans and an increase in chitin synthesis were detected; (4) Conclusions: Helium-CAP caused morphological and topographic alterations in cells and affected the cell wall polysaccharide content.
(1) 背景：先前的研究报告表明，冷等离体等离子体（CAP）对 的抑制作用很有前景。然而，CAP 对真菌细胞的确切作用机制仍知之甚少。本研究旨在通过评估其结构和生化组成的变化，阐明 CAP 对 细胞壁的影响；(2) 方法：用氦气 CAP 处理的 细胞通过原子力显微镜（AFM）和傅里叶变换红外光谱（FTIR）进行分析，以检测真菌细胞壁的形态、形貌和生化变化。为了进行比较，还分析了用卡泊芬净处理的细胞；(3) 结果：在 CAP 暴露后，观察到细胞发生了明显的形态和形貌变化，如粗糙度增加和形状改变。检测到的变化与用卡泊芬净处理后观察到的变化相似。主要的生化变化发生在多糖含量上，检测到葡聚糖含量总体下降，壳聚糖合成增加；(4) 结论：氦气 CAP 导致 细胞发生形态和形貌改变，并影响细胞壁多糖含量。
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