Bekiaris Georgios, Tagkouli Dimitra, Koutrotsios Georgios, Kalogeropoulos Nick, Zervakis Georgios I
Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, 11855 Athens, Greece.
Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University of Athens, 17676 Athens, Greece.
Foods. 2020 Apr 24;9(4):535. doi: 10.3390/foods9040535.
Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy was used to monitor the infrared absorption spectra of 79 mushroom samples from 29 , and strains cultivated on wheat straw, grape marc and/or by-products of the olive industry. The spectroscopic analysis provided a chemical insight into the mushrooms examined, while qualitative and quantitative differences in regions related to proteins, phenolic compounds and polysaccharides were revealed among the species and substrates studied. Moreover, by using advanced chemometrics, correlations of the recorded mushrooms' spectra versus their content in glucans and ergosterol, commonly determined through traditional analytical techniques, allowed the development of models predicting such contents with a good predictive power (: 0.80-0.84) and accuracy (low root mean square error, low relative error and representative to the predicted compounds spectral regions used for the calibrations). Findings indicate that FTIR spectroscopy could be exploited as a potential process analytical technology tool in the mushroom industry to characterize mushrooms and to assess their content in bioactive compounds.
衰减全反射傅里叶变换红外光谱（ATR-FTIR）用于监测来自29个种、变种和菌株的79个蘑菇样品在小麦秸秆、葡萄皮渣和/或橄榄油工业副产品上培养时的红外吸收光谱。光谱分析为所检测的蘑菇提供了化学见解，同时在所研究的物种和底物之间揭示了与蛋白质、酚类化合物和多糖相关区域的定性和定量差异。此外，通过使用先进的化学计量学，将记录的蘑菇光谱与其葡聚糖和麦角甾醇含量（通常通过传统分析技术测定）进行关联，从而开发出具有良好预测能力（R²：0.80 - 0.84）和准确性（低均方根误差、低相对误差以及校准所用预测化合物光谱区域具有代表性）的预测此类含量的模型。研究结果表明，FTIR光谱可作为蘑菇产业中一种潜在的过程分析技术工具，用于表征蘑菇并评估其生物活性化合物含量。
