Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
Da Chan Bay Customs People's Republic of China, Shenzhen, 518102, China.
Arch Virol. 2023 Dec 12;169(1):8. doi: 10.1007/s00705-023-05927-y.
A method for separation of spring viraemia of carp virus (SVCV) from large-volume samples using immunomagnetic beads (IMBs) coated with a polyclonal antibody against SVCV was developed. The optimum amount of IMBs was 2 mg in 100 mL. After IMB treatment, the detection limit of SVCV in reverse transcription quantitative PCR (RT-qPCR) was 10 times the 50% tissue culture infectious dose per mL in 100-mL samples. The concentration of viral RNA extracted from SVCV that had been separated using IMBs was 5.18 × 10-fold higher than that of the unseparated SVCV. When fish samples were tested, the concordance rates of the IMBs/RT-qPCR and RT-qPCR were 100% and 67.5%, respectively.
采用免疫磁珠（IMB）分离试剂盒，建立了一种从大量样品中分离鲤春病毒血症病毒（SVCV）的方法。该试剂盒的最佳工作浓度为 2mg/100mL。经 IMB 处理后，逆转录定量 PCR（RT-qPCR）检测的灵敏度比常规 100mL 样品的 50%组织培养半数感染剂量（TCID50）提高了 10 倍。从 IMB 分离的 SVCV 中提取的病毒 RNA 浓度比未经分离的 SVCV 高 5.18×10 倍。当对鱼样进行检测时，免疫磁珠/RT-qPCR 方法和 RT-qPCR 方法的符合率分别为 100%和 67.5%。
