Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China.
Fisheries College, Ocean University of China, Yushan Road 5, Qingdao, 266003, China.
Environ Sci Pollut Res Int. 2024 Jan;31(3):3495-3511. doi: 10.1007/s11356-023-31404-9. Epub 2023 Dec 12.
With the accelerated modernization of agriculture and industry, sulfides have been released into the environment as a by-products of various production processes. Elevated levels of sulfide pose a threat to organisms' health and disrupt ecosystem equilibrium. This study successfully isolated two highly efficient sulfur-oxidizing strains, namely Pseudomonas aeruginosa GHWS3 and Sphingobacterium sp. GHWS5. Neither strain exhibited hemolytic activity or pathogenicity. Additionally, GHWS3 inhibited the common aquaculture pathogen Vibrio anguillarum, while GHWS5 exhibited inhibitory effects against Vibrio harveyi. GHWS3 and GHWS5 demonstrated effective removal of sulfide under the following conditions: temperature range of 20-40 °C, pH level of 4.5-8.5, salinity range of 0-50‰, C/N ratio of 5-15, and sulfide concentration of 20-200 mg/L. By amplifying the key functional genes of the sulfur-oxidizing Sox and rDsr systems in both GHWS3 and GHWS5 strains, potential desulfurization pathways were analyzed. Furthermore, both strains displayed high efficiency in removing sulfides from actual aquaculture pond substrate mixtures. The findings of this study provide two promising candidate strains for sulfides removal from farm tailwater, industrial wastewater, and domestic wastewater.
随着农业和工业的现代化加速，硫化物作为各种生产过程的副产品被释放到环境中。高浓度的硫化物对生物的健康构成威胁，并破坏生态系统平衡。本研究成功分离出两株高效硫氧化菌株，即铜绿假单胞菌 GHWS3 和鞘氨醇单胞菌 GHWS5。这两株菌均不表现溶血活性或致病性。此外，GHWS3 抑制了常见的水产养殖病原菌鳗弧菌，而 GHWS5 对哈维氏弧菌表现出抑制作用。GHWS3 和 GHWS5 在以下条件下能有效去除硫化物：温度范围 20-40°C，pH 值 4.5-8.5，盐度范围 0-50‰，C/N 比 5-15，硫化物浓度 20-200mg/L。通过扩增 GHWS3 和 GHWS5 两株菌中硫氧化 Sox 和 rDsr 系统的关键功能基因，分析了潜在的脱硫途径。此外，两株菌均能高效去除实际水产养殖池塘基质混合物中的硫化物。本研究为从养殖尾水、工业废水和生活污水中去除硫化物提供了两种有前途的候选菌株。
