Possati L, Collesi A, Rosciani C, Rubini C
Oncology. 1986;43(6):368-71. doi: 10.1159/000226404.
Primary fetal rat fibroblasts (FRF cells) were transfected with DNA extracted from BKV-transformed hamster kidney cells at low passage after transformation (HKBKlp cells) and at high passage after transformation (HKBKhp cells). Two transformed rat cell lines were obtained, FRF-DNA-HKBKlp cells and FRF-DNA-HKBKhp cells, transformed by DNA extracted from HKBKlp cells and HKBKhp cells, respectively. They were characterized by a number of biological properties and by tumorigenicity and metastatic ability in newborn hamsters. The two transformed FRF cell lines exhibited exactly inverted properties in comparison with HKBK cells, in particular FRF-DNA-HKBKlp cells showed a low level of nuclear T antigen and a diffuse appearance of TSTA at the cell surface, whereas FRF-DNA-HKBKhp cells demonstrated a high level of T antigen and the capping of TSTA in the cell membrane. The tumorigencity and the metastatic ability were greater with FRF-DNA-HKBKlp cells, while FRF-DNA-HKBKhp cells appeared to be less tumorigenic and metastatic in newborn hamsters.
将来自转化后低传代的BKV转化仓鼠肾细胞（HKBKlp细胞）和转化后高传代的BKV转化仓鼠肾细胞（HKBKhp细胞）提取的DNA转染原代大鼠胚胎成纤维细胞（FRF细胞）。获得了两个转化的大鼠细胞系，即分别由HKBKlp细胞和HKBKhp细胞提取的DNA转化而成的FRF-DNA-HKBKlp细胞和FRF-DNA-HKBKhp细胞。通过多种生物学特性以及在新生仓鼠中的致瘤性和转移能力对它们进行了表征。与HKBK细胞相比，这两个转化的FRF细胞系表现出完全相反的特性，特别是FRF-DNA-HKBKlp细胞显示出低水平的核T抗原以及细胞表面TSTA的弥散外观，而FRF-DNA-HKBKhp细胞则表现出高水平的T抗原以及细胞膜中TSTA的封帽现象。FRF-DNA-HKBKlp细胞的致瘤性和转移能力更强，而FRF-DNA-HKBKhp细胞在新生仓鼠中似乎致瘤性和转移性较低。
