Bélanger P M, Grech-Bélanger O
Drug Metab Dispos. 1979 May-Jun;7(3):171-5.
Washed microsomes from rabbit liver reduced 1-nitrosoadamantane to N-hydroxy-1-aminoadamantane in the presence of a cofactor solution under aerobic conditions; no further reduction of the hydroxylamino metabolite to 1-aminoadamantane (amantadine) occurred. Reduced pyridine nucleotide cofactors are needed for the metabolic reduction. The rate of formation of N-hydroxy-1-aminoadamantane depended upon the microsomal protein content, the time of incubation and the concentration of 1-nitrosoadamantane incubated. The metabolic reduction occurred in air as well as under nitrogen or carbon monoxide. Cupric chloride, mercuric chloride, cysteamine, FAD, and FMN decreased significantly the C-nitroso reductase. The properties of the C-nitroso reductase differed from those of other microsomal reductive pathways.
在有氧条件下，存在辅因子溶液时，兔肝洗涤微粒体可将1-亚硝基金刚烷还原为N-羟基-1-氨基金刚烷；羟基氨基代谢产物不会进一步还原为1-氨基金刚烷（金刚烷胺）。代谢还原需要还原型吡啶核苷酸辅因子。N-羟基-1-氨基金刚烷的形成速率取决于微粒体蛋白含量、孵育时间以及所孵育的1-亚硝基金刚烷的浓度。代谢还原在空气中以及氮气或一氧化碳下均可发生。氯化铜、氯化汞、半胱胺、黄素腺嘌呤二核苷酸（FAD）和黄素单核苷酸（FMN）显著降低了C-亚硝基还原酶的活性。C-亚硝基还原酶的特性与其他微粒体还原途径不同。
