Chen Tao, Li Baoxia, Zheng Kangdi, Liu Yan, Zhang Zhao, Hu Huimei, Qian Guoqiang, Jiang Jianwei
Center of Human Microecology Engineering and Technology of Guangdong Province, Guangdong Longsee Biomedical Corporation, Guangzhou, 510535, Guangdong, China.
School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, China.
Curr Microbiol. 2023 Dec 13;81(1):38. doi: 10.1007/s00284-023-03525-4.
Lactobacillus paracasei (L. paracasei), a common probiotic lactobacillus, has important functions in the food industry and human health. However, different strains of L. paracasei inevitably show differences in activity and colonization resistance, leading to differentiation in their functions, as well as their physical or chemical properties. The purpose of this study was to evaluate the characteristics of L. paracasei R3 (L.p R3) isolated from healthy human feces and determine whether the criteria for edible probiotics is met. The hemolysis type, biofilm-forming ability, antibiotic susceptibility, toxicity, and effective activity of L.p R3 were determined by establishing its probiotic activity traits in vitro and in vivo. The results showed that L.p R3 had a moderate biofilm formation ability, was sensitive to 11 antibiotics, was resistant to eight antibiotics, and was not hemolytic. The culture characteristics, morphology, and biochemical responses of the strain were consistent with the seed batch characteristics. In toxicity assays, L.p R3-fed mice showed no abnormalities in body weight, growth, or various organs. Additionally, L.p R3 was found to be effective in the prevention and treatment of colorectal cancer. In conclusion, our results revealed that L.p R3 has potential value as an edible probiotic without toxic side effects and alleviated the tumor progression of colorectal cancer in mice.
副干酪乳杆菌是一种常见的益生菌，在食品工业和人类健康方面具有重要作用。然而，不同的副干酪乳杆菌菌株在活性和定植抗性方面不可避免地存在差异，导致其功能以及物理或化学性质出现分化。本研究的目的是评估从健康人粪便中分离出的副干酪乳杆菌R3（L.p R3）的特性，并确定其是否符合食用益生菌的标准。通过在体外和体内建立L.p R3的益生菌活性特征，测定了其溶血类型、生物膜形成能力、抗生素敏感性、毒性和有效活性。结果表明，L.p R3具有中等生物膜形成能力，对11种抗生素敏感，对8种抗生素耐药，且不溶血。该菌株的培养特性、形态和生化反应与种子批特征一致。在毒性试验中，喂食L.p R3的小鼠在体重、生长或各个器官方面均未出现异常。此外，发现L.p R3对预防和治疗结直肠癌有效。总之，我们的结果表明，L.p R3作为一种无毒性副作用的食用益生菌具有潜在价值，并可缓解小鼠结直肠癌的肿瘤进展。
