Hu Juan, Ren Wubo, Liu Han, Xie Cong, Li Dandan, Yang Liping, Liao Fangli, Jiang Linshan, Pu Qinli, Chen Weixian
Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, No. 74 Linjiang Road, Yuzhong, Chongqing, 400010, People's Republic of China.
The Experimental Medicine Center, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing, 400060, People's Republic of China.
Mikrochim Acta. 2023 Dec 13;191(1):27. doi: 10.1007/s00604-023-06098-y.
The current use of the single serum biomarker α-fetoprotein (AFP) in clinical practice has limitations in terms of specificity and sensitivity. We propose a strategy that combines antigen capture polymerase chain reaction (AC-PCR), lateral flow assay (LFA), and electrochemical biosensors to detect both AFP and circulating tumor cells (CTCs) in liver cancer serum. First, we used the AC-PCR technique to achieve target separation, purification, signal conversion, and amplification, eliminating target heterogeneity. Then, we achieved rapid results through the LFA and electrochemical biosensor platforms. As a result, the proposed assay has limits of 5 cells/mL for CTCs and 5 µg/L for AFP. The proposed method was applied effectively to simulated blood samples. This method has the potential to play a role in early liver cancer and provide a potential application for the diagnosis and precision treatment of liver cancer.
目前临床实践中单一血清生物标志物甲胎蛋白（AFP）的应用在特异性和敏感性方面存在局限性。我们提出了一种将抗原捕获聚合酶链反应（AC-PCR）、侧向流动分析（LFA）和电化学生物传感器相结合的策略，以检测肝癌血清中的AFP和循环肿瘤细胞（CTC）。首先，我们使用AC-PCR技术实现靶标分离、纯化、信号转换和扩增，消除靶标异质性。然后，我们通过LFA和电化学生物传感器平台实现了快速检测结果。结果，所提出的检测方法对CTC的检测限为5个细胞/毫升，对AFP的检测限为5微克/升。所提出的方法有效地应用于模拟血样。该方法有潜力在早期肝癌中发挥作用，并为肝癌的诊断和精准治疗提供潜在应用。
