Zhang Jinzha, Liu Xuan, Liu Huaxiao, Wang Jingzhi, Zhang Yawen, Zhao Wenbo
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China.
National and Local Joint Engineering Research Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China.
Anal Chim Acta. 2022 Sep 15;1226:340251. doi: 10.1016/j.aca.2022.340251. Epub 2022 Aug 11.
Abnormal glutathione (GSH) levels are directly related to specific diseases. Therefore, the highly sensitive detection of GSH has attracted extensive attention of researchers. Here, we reported a novel electrochemiluminescence (ECL) biosensor platform based on AgGaS quantum dots (AGS QDs) for sensitive detection of GSH released by living cancer cells. Firstly, the preparation and photoluminescence of AGS QDs were studied. Then, ECL biosensor based on AGS QDs was constructed and applied to the detection of GSH released by MCF-7 cells. The experimental results showed that the ECL biosensor can sensitively detect GSH directly released by living cancer cells in situ. This work not only provides a promising choice for developing ECL with low toxicity and good biocompatibility, but also can be extended to detect other molecules released by cells, which is conducive to the in-depth study of pathophysiology.
异常的谷胱甘肽（GSH）水平与特定疾病直接相关。因此，对GSH的高灵敏度检测引起了研究人员的广泛关注。在此，我们报道了一种基于AgGaS量子点（AGS QDs）的新型电化学发光（ECL）生物传感器平台，用于灵敏检测活癌细胞释放的GSH。首先，研究了AGS QDs的制备和光致发光。然后，构建了基于AGS QDs的ECL生物传感器，并将其应用于检测MCF-7细胞释放的GSH。实验结果表明，该ECL生物传感器能够原位灵敏地检测活癌细胞直接释放的GSH。这项工作不仅为开发低毒性和良好生物相容性的ECL提供了一个有前景的选择，而且还可以扩展到检测细胞释放的其他分子，这有利于病理生理学的深入研究。
