Department of Zoology, Biomedical Technology, Human Genetics and Wildlife Conservation and Biology, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad, Gujarat 380009, India.
Department of Chemistry, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad, Gujarat 380009, India.
J Chromatogr A. 2024 Jan 11;1714:464555. doi: 10.1016/j.chroma.2023.464555. Epub 2023 Dec 2.
The objective of this study was to evaluate the efficiency of nematodes in zooremediation of chlorpyrifos (CPF), an organophosphate pesticide. The nematode population Acrobeloides maximus (A. maximus) was employed for bioremediation, converting CPF into non-toxic residues. Optimal growth conditions for mass production of A. maximus were achieved by maintaining a temperature of 25 °C, pH 8, and supplementing the culture medium with plant nutrients. The nematodes were then immobilized within sodium alginate beads. The efficacy of the degradation process was assessed using various analytical techniques, including UV-Visible spectroscopy, HPTLC, FTIR, and LC-MS, confirming the successful breakdown of CPF. The bioreactor demonstrated a complete degradation efficiency of CPF exceeding 99%. Additionally, LC-MS analysis was conducted to elucidate the degradation pathway based on the formation of intermediates. These results underscore the potential of A. maximus as a sustainable organism for addressing environmental contamination arising from CPF pesticide.
本研究旨在评估线虫在毒死蜱（CPF），一种有机磷农药的生物修复中的效率。采用线虫种群巨型嗜热线虫（A. maximus）进行生物修复，将 CPF 转化为无毒残留物。通过将温度维持在 25°C、pH 值为 8，并在培养基中添加植物营养素，实现了 A. maximus 大规模生产的最佳生长条件。然后将线虫固定在海藻酸钠珠中。使用各种分析技术，包括紫外可见光谱、HPTLC、FTIR 和 LC-MS，评估降解过程的效果，证实 CPF 成功分解。生物反应器表现出超过 99%的 CPF 完全降解效率。此外，还进行了 LC-MS 分析，根据中间产物的形成阐明了降解途径。这些结果突出了 A. maximus 作为一种可持续生物体的潜力，可用于解决 CPF 农药引起的环境污染问题。
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