Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Material Science and Engineering, State Key Laboratory of Fine Chemicals, Shenzhen University, Shenzhen 518060, PR China.
School of Physics, The University of Sydney, NSW 2006, Australia.
J Hazard Mater. 2023 Jun 15;452:131177. doi: 10.1016/j.jhazmat.2023.131177. Epub 2023 Mar 15.
Chlorpyrifos (CPF) as a classic organophosphorus pesticide has been widely used in agricultural applications to control insects and worms. CPF in the environment can cause deaths of diverse kinds of aquatic organism and bring a high risk to human health. Therefore, the development of effective analytical method for CPF is of great importance. In this work, a novel dual-mode albumin (ALB)-based supramolecular probe FD@ALB was designed and prepared for rapid detection of CPF in the environment. The limit of detection is 0.57 μM (∼ 0.2 ppm) with a wider detection range up to 200 μM, which is satisfactory for application. The sensing mechanism can be ascribed to CPF-induced phosphorylation of ALB, thus leading to a change in the binding microenvironment of FD dye. Moreover, the paper-based test strips were used in conjunction with the FD@ALB, realizing the portable detection of CPF. This method was demonstrated to be suitable for on-site detection of CPF in various kinds of environmental samples, including water, soil, and food samples, with the aid of a smartphone. To the best of our knowledge, this is the first analytical method achieving a combination of the rapid and ratiometric detection of CPF in the environment.
毒死蜱（CPF）作为一种经典的有机磷农药，已广泛应用于农业领域以防治昆虫和蠕虫。环境中的 CPF 会导致各种水生生物死亡，并对人类健康构成高风险。因此，开发有效的 CPF 分析方法非常重要。在这项工作中，设计并制备了一种新型基于双模式白蛋白（ALB）的超分子探针 FD@ALB，用于快速检测环境中的 CPF。其检测限为 0.57 μM（约 0.2 ppm），检测范围更宽，可达 200 μM，可满足实际应用的需求。传感机制可归因于 CPF 诱导的 ALB 磷酸化，从而导致 FD 染料结合微环境发生变化。此外，还结合 FD@ALB 使用了纸质测试条，实现了 CPF 的便携检测。该方法已被证明适用于各种环境样品（包括水、土壤和食物样品）中 CPF 的现场检测，并且借助智能手机可实现。据我们所知，这是第一种实现环境中 CPF 的快速和比率检测相结合的分析方法。
